glycogen and Hodgkin-Disease

In order to investigate the disordered maturation of mononuclear phagocytes previously found in Hodgkin's disease, integrating microdensitometry was used to quantitate changes in seven cytochemical constituents (NADH dehydrogenase, succinate dehydrogenase, acid phosphatase, alpha-naphthyl butyrate esterase, DNA, RNA and glycogen) of developing macrophages from 19 patients and 19 normal subjects. Individual cells were studied at intervals over six days of suspension culture; the results were subjected to analysis of variance. In both groups, all the constituents studied except DNA showed highly significant increases over the period of culture. Consistently lower levels of alpha-naphthyl butyrate esterase (approximately 65%) and increased levels of glycogen were present in the Hodgkin's group. The results show that (1) maturational changes occur in the cytochemical constituents of developing macrophages of Hodgkin's disease, and (2) there are disturbances affecting the specific enzyme alpha-naphthyl butyrate esterase and glycogen that are likely to have profound implications for host defense mechanisms.

Topics: Acid Phosphatase; Adult; Analysis of Variance; Carboxylic Ester Hydrolases; Cell Differentiation; DNA, Neoplasm; Female; Glycogen; Histocytochemistry; Hodgkin Disease; Humans; Macrophages; Male; NADH Dehydrogenase; RNA, Neoplasm; Succinate Dehydrogenase

In exsudate cells separated from serous body cavities of 29 tumour patients and 30 patients with inflammatory and congestive effusion in cardiac failure or liver cirrhosis respectively the activities of acid and alkaline phosphatase were determined. In addition to sudanophilia the cell content of glycogen and that of ribonucleinic acid were evaluated. By means of cytochemical findings it could be found that an increase of unspecific esterase, acid phosphatase and ribonucleic acid in atypical cells points to a malignous ethiology of the exudate.

Topics: Acid Phosphatase; Alkaline Phosphatase; Ascitic Fluid; Esterases; Exudates and Transudates; Glycogen; Heart Failure; Histocytochemistry; Hodgkin Disease; Humans; Leukemia; Liver Cirrhosis; Lymphoma, Large B-Cell, Diffuse; Neoplasms; Peritonitis; Pleural Effusion; Pleurisy; RNA

Except for tumors clearly producing immunoglobulin (e.g. plasma-cytoma), the different classes of malignant lymphomas do not correlate with a constant surface immunoglobulin pattern. Beside a prevailing IgM-surface type in a high number of different lymphoma classes even T-like or O-cells occur in most tumor types provided they are studied in a sufficient number. The possibility of dedifferentiation of immunologic cell qualities has to be envisaged. In this context intracellular lambda-chain crystals occurring in chronic lymphocytic leukaemia may provide a morphological hallmark of anaplastic deterioration of immunoglobulin synthesis. - The ambiguous significance of PAS-positive inclusions and virus-like microtubular complexes in lymphoma cells is discussed. - In conclusion the recent discovery of hairy cell leukaemia provides a good example of the value of nomenclatures based on empiricism rather than on short-lived theories.

Topics: B-Lymphocytes; Cell Membrane; Fluorescent Antibody Technique; Glycogen; Histocytochemistry; Hodgkin Disease; Humans; Immune Adherence Reaction; Immunoglobulins; Inclusion Bodies; Inclusion Bodies, Viral; Leukemia, Lymphoid; Leukemia, Plasma Cell; Lymphocytes; Lymphoma, Follicular; T-Lymphocytes; Terminology as Topic

Electron microscopy was performed on two CNS lymphomas, one primary and the second a skull metastasis from a brain primary lymphoma. The former revealed a tumor perivascularly arranged, which was composed of rounded cells having no specialized organellae. Biopsies from the primary and metastasis of the second case revealed a predominance of rounded cells with scanty cytoplasm, another population of cells with phagocytic activity and a third group with elongated ctyoplasmic processes which stain positively for microglia. By electron microscopy some of these processes resembled neurites or oligondendroglia, while others suggested an astrocytic appearance.

Topics: Adult; Blood Vessels; Brain Neoplasms; Cell Differentiation; Glycogen; Hodgkin Disease; Humans; Male; Microtubules; Middle Aged; Neoplasm Metastasis; Neuroglia; Organoids; Phagocytes; Skull Neoplasms

Topics: Adenocarcinoma, Mucinous; Cecum; Cell Nucleolus; DNA, Neoplasm; Glycogen; Histocytochemistry; Hodgkin Disease; Humans; Lymphoma, Large B-Cell, Diffuse; Neoplasm Proteins; RNA, Neoplasm; Staining and Labeling

Topics: Glycogen; Hodgkin Disease; Humans; In Vitro Techniques; Leukemia, Lymphoid; Lymphocytes; Lymphoma, Non-Hodgkin; Methods; Radiation Effects

Topics: Acute Disease; Adolescent; Adult; Biopsy; Cytoplasm; Demyelinating Diseases; Female; Glycogen; Hodgkin Disease; Humans; Leukemia; Male; Microscopy; Microscopy, Electron; Middle Aged; Nerve Degeneration; Polyneuropathies; Schwann Cells; Sural Nerve; Vinblastine; Vinca Alkaloids; Vincristine

Topics: Acid Phosphatase; Culture Techniques; Esterases; Glycogen; Hodgkin Disease; Humans; Lectins; Leukemia; Leukemia, Lymphoid; Lymphocyte Activation; Lymphocytes; Lymphoma; Lymphoma, Non-Hodgkin; Succinate Dehydrogenase

Topics: Diagnosis, Differential; Glycogen; Histocytochemistry; Hodgkin Disease; Humans; Neoplasms

Topics: Glycogen; Histocytochemistry; Hodgkin Disease; Humans; In Vitro Techniques; Lipids; Lymph Nodes; Lymphoma, Large B-Cell, Diffuse; Phosphoric Monoester Hydrolases; RNA

The lymphocyte glycogen content has been measured semi-quantitatively by staining peripheral blood films by the periodic-acid-Schiff (P.A.S.) technique. The high values reported in chronic lymphatic leukaemia, lymphosarcoma, Hodgkin's disease, and infectious mononucleosis have been confirmed. High values have also been found in cases with chronic suppuration, ulcerative colitis, myelomatosis, and epithelial malignancies. Our results suggest that a high lymphocyte glycogen content may be produced by several different mechanisms and that the test is of little value in differential diagnosis.

Topics: Colitis, Ulcerative; Diagnosis, Differential; Glycogen; Hodgkin Disease; Humans; Leukemia, Lymphocytic, Chronic, B-Cell; Lymphocytes; Multiple Myeloma; Staining and Labeling