glycogen and Hand-Dermatoses

Fluorescence of pathogenic fungi has been previously shown when hematoxylin and eosin-stained sections are examined under a fluorescent microscope. We hypothesize that this phenomenon could aid in the evaluation of nail specimens for onychomycosis.. Forty-eight routinely stained nail sections of periodic acid-Schiff (PAS)-positive onychomycosis, along with 23 PAS-negative control specimens with a clinical diagnosis of onychomycosis, were studied under a fluorescent microscope to determine the clinical usefulness of this technique.. In most cases, fluorescence of fungal organisms was noted. Fungi were identified by their tubular or annular shapes with fluorescence surrounding them. The sensitivity and specificity of the method were 96 and 90%, respectively. In some cases, it was difficult to identify the fungi because of the relative paucity of organisms, weak fluorescence and high background fluorescence of eosinophilic nail keratin.. We conclude that fluorescence microscopy can be used as a rapid screening tool for identification of fungi in nail specimens.

Topics: Adolescent; Adult; Aged; Aged, 80 and over; Arthrodermataceae; Female; Fluorescence; Foot Dermatoses; Glycogen; Hand Dermatoses; Humans; Male; Microscopy, Fluorescence; Middle Aged; Onychomycosis; Periodic Acid-Schiff Reaction; Sensitivity and Specificity; Young Adult

This paper deals with the histological and ultrastructural findings in a case of the Sézary syndrome. The striking nuclear and cytoplasmic features of the Sézary cell are illustrated, and the similarities of this cell to the mycosis fungoides cell are once more stressed.

Topics: Cell Nucleus; Cytoplasm; Dermatitis, Exfoliative; Edema; Foot Dermatoses; Glycogen; Golgi Apparatus; Hand Dermatoses; Histocytochemistry; Humans; Keratosis; Lymphatic Diseases; Male; Microscopy, Electron; Middle Aged; Mitochondria; Mycosis Fungoides; Pruritus; Skin; Staining and Labeling

Topics: Alkaline Phosphatase; Anemia, Aplastic; Blood Cells; Bone Marrow; Bone Marrow Cells; Bone Marrow Diseases; Dermatitis, Exfoliative; Edema; Esterases; Foot Dermatoses; Glucuronidase; Glycogen; Hand Dermatoses; Histocytochemistry; Humans; Keratosis; Leukemia, Lymphoid; Lymphatic Diseases; Periodic Acid; Pruritus