glycogen and Fibrosarcoma

Mice bearing IL-1beta-secreting tumor were used to study the chronic effect of IL-1beta on glucose metabolism. Mice were injected with syngeneic tumor cells transduced with the human IL-1beta gene. Serum IL-1beta levels increased exponentially with time. Secretion of IL-1beta from the developed tumors was associated with decreased food consumption, reduced body weight, and reduced blood glucose levels. Body composition analysis revealed that IL-1beta caused a significant loss in fat tissue without affecting lean body mass and water content. Hepatic phosphoenolpyruvate carboxykinase and glucose-6-phosphatase activities and mRNA levels of these enzymes were reduced, and 2-deoxy-glucose uptake by peripheral tissues was enhanced. mRNA levels of glucose transporters (Gluts) in the liver were determined by real-time PCR analysis. Glut-3 mRNA levels were up-regulated by IL-1beta. Glut-1 and Glut-4 mRNA levels in IL-1beta mice were similar to mRNA levels in pair-fed mice bearing nonsecreting tumor. mRNA level of Glut-2, the major Glut of the liver, was down-regulated by IL-1beta. We concluded that both decreased glucose production by the liver and enhanced glucose disposal lead to the development of hypoglycemia in mice bearing IL-1beta-secreting tumor. The observed changes in expression of hepatic Gluts that are not dependent on insulin may contribute to the increased glucose uptake.

Topics: Animals; Anorexia; Blood Glucose; Body Composition; Body Weight; C-Peptide; Cell Line, Tumor; Eating; Female; Fibrosarcoma; Gluconeogenesis; Glucose; Glucose-6-Phosphatase; Glycogen; Humans; Hypoglycemia; Hypoglycemic Agents; Insulin; Interleukin-1; Leptin; Liver; Mice; Mice, Inbred C57BL; Monosaccharide Transport Proteins; Neoplasm Transplantation; Protein Serine-Threonine Kinases; RNA, Messenger

Mice bearing interleukin-6 (IL-6)-secreting tumor were used to study the chronic effect of IL-6 on carbohydrate metabolism. Mice were injected with allogeneic tumor cells transduced with the murine IL-6 gene. Serum IL-6 levels were correlated exponentially with tumor weight. Secretion of IL-6 from the developed tumors was associated with decreased food consumption, reduced body weight, and reduced blood glucose levels. Insulin levels did not change, and 2-deoxyglucose uptake was not affected in most tissues examined. A significant increase of 2-deoxyglucose uptake was measured in the liver. Glycogen content in the liver determined 0, 6, 12, and 18 days after tumor inoculation was 42, 23, 12, and 3 mg/g, respectively. The activity of phosphoenolpyruvate carboxykinase was not affected. The activity of glucose-6-phosphatase (G-6-Phase) determined 6, 12, and 18 days after tumor injection was 84, 70, and 50% of G-6-Pase activity in pair-fed mice bearing nonsecreting tumors, respectively. G-6-Pase mRNA levels were markedly reduced due to inhibition of G-6-Pase gene transcriptional rate.

Topics: Animals; Blood Glucose; Body Weight; Deoxyglucose; Eating; Female; Fibrosarcoma; Gene Expression; Glucose-6-Phosphatase; Glycogen; Insulin; Interleukin-6; Liver; Mice; Mice, Inbred C57BL; Neoplasm Transplantation; Transduction, Genetic

Topics: Animals; Antibiotics, Antineoplastic; Fibrosarcoma; Glucose; Glycogen; Lactates; Male; Mice; Mice, Inbred Strains; Muscles; Pyruvates; Sarcoma, Experimental

Topics: Alkaline Phosphatase; Biopsy; Bone Cysts; Bone Diseases; Bone Neoplasms; Chondroma; Chondrosarcoma; Clinical Enzyme Tests; Diagnosis, Differential; Femoral Neoplasms; Fibrosarcoma; Giant Cell Tumors; Glycogen; Hemangioma; Hemangiosarcoma; Histocytochemistry; Humans; Ilium; Lymphoma, Large B-Cell, Diffuse; Multiple Myeloma; Neoplasm Metastasis; Osteoma; Osteoma, Osteoid; Osteomyelitis; Osteosarcoma; Ribs; Sarcoma, Ewing; Tibia; Trephining; Tuberculosis, Osteoarticular

Topics: Adipose Tissue; Cartilage; Chondrosarcoma; Chordoma; Connective Tissue; Female; Fibroma; Fibrosarcoma; Glycogen; Hemangioma; Humans; Leiomyoma; Leiomyosarcoma; Lipoma; Liposarcoma; Muscle, Smooth; Neoplasms, Connective Tissue; Neoplasms, Muscle Tissue; Neoplasms, Nerve Tissue; Neurilemmoma; Neurofibroma; Sarcoma; Sarcoma, Synovial; Synovial Membrane; Uterus

Topics: Alkaline Phosphatase; Cadmium; Electron Transport Complex IV; Fibrosarcoma; Glycogen; Humans; Lactates; Male; Methylcholanthrene; Neoplasm Transplantation; Neoplasms; Nitrogen; Pharmacology; Rats; Research; RNA; RNA, Neoplasm; Testicular Neoplasms; Toxicology