glycogen and Femoral-Neoplasms

Four small round cell malignant tumors of bone occurring in children are described. There was no catecholamine secretion and the clinical, radiologic, and biopsy diagnosis in each was Ewing's sarcoma. Glycogen was sparse both on imprints and in tissue sections. The tumors, when extensively sampled, had areas of a lobular growth pattern and Homer Wright rosettes. The rosettes were always focal and varied in complexity from case to case; they were rudimentary in one instance and markedly fibrillar in the most obvious instance. Neuron-specific enolase was demonstrated in tissue sections and in longterm cell cultures from three of the tumors. The cultured cells put out moderately long beaded processes in serum-free medium but had no catecholamine fluorescence. Electron microscopy of the tumor rosettes and the cultured cells showed processes containing aggregates of microtubules and only one case had rare neurosecretory granules. This study suggests that some small round cell tumors of bone and soft tissue in children, which present as Ewing's sarcoma, are neuroectodermal in nature.

Topics: Bone Neoplasms; Calcaneus; Child; Child, Preschool; Female; Femoral Neoplasms; Glycogen; Humans; Male; Microscopy, Electron; Neuroblastoma; Neuroectodermal Tumors, Primitive, Peripheral; Phosphopyruvate Hydratase; Rosette Formation; Sarcoma, Ewing; Scapula; Staining and Labeling

Topics: Adult; Chondrosarcoma; Femoral Neoplasms; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Male; Microscopy, Electron; Vacuoles

The authors report five cases treated by wide resection. Pathological studies were made of the whole of the lesion. Three tumours were situated at the upper end of the femur, one at the upper end of the humerus and one at the level of the tibial plateau. The specific features were localisation in an epiphysis and histological areas of clear cells whose cytoplasm contained glycogen. From the anatomical and radiological points of view, these tumours were like chondroblastomata but their progress was more like that of chondrosarcomata. The author concludes that they should be distinguished from both types of tumour.

Topics: Adult; Bone Neoplasms; Chondroblastoma; Chondrosarcoma; Diagnosis, Differential; Epiphyses; Female; Femoral Neoplasms; Glycogen; Humans; Humerus; Male; Microscopy, Electron; Middle Aged; Osteogenesis; Radiography; Tibia

Topics: Adult; Aged; Bone Neoplasms; Cartilage, Articular; Cell Nucleolus; Cell Nucleus; Chondrosarcoma; Endoplasmic Reticulum; Female; Femoral Neoplasms; Glycogen; Golgi Apparatus; Humans; Humerus; Inclusion Bodies; Male; Microscopy, Electron; Middle Aged; Mitochondria; Scapula; Sternum; Thoracic Neoplasms

Topics: Adult; Aged; Cell Nucleolus; Cell Nucleus; Chondrosarcoma; Chromatin; Cytoplasm; Endoplasmic Reticulum; Female; Femoral Neoplasms; Glycogen; Humans; Humerus; Laryngeal Neoplasms; Lipids; Male; Microscopy, Electron; Middle Aged; Prognosis; Sacrum; Tibia

Topics: Alkaline Phosphatase; Biopsy; Bone Cysts; Bone Diseases; Bone Neoplasms; Chondroma; Chondrosarcoma; Clinical Enzyme Tests; Diagnosis, Differential; Femoral Neoplasms; Fibrosarcoma; Giant Cell Tumors; Glycogen; Hemangioma; Hemangiosarcoma; Histocytochemistry; Humans; Ilium; Lymphoma, Large B-Cell, Diffuse; Multiple Myeloma; Neoplasm Metastasis; Osteoma; Osteoma, Osteoid; Osteomyelitis; Osteosarcoma; Ribs; Sarcoma, Ewing; Tibia; Trephining; Tuberculosis, Osteoarticular

Topics: Adolescent; Calcification, Physiologic; Calcinosis; Cell Membrane; Cell Nucleus; Endoplasmic Reticulum; Female; Femoral Neoplasms; Glycogen; Golgi Apparatus; Humans; Hydroxyapatites; Lipid Metabolism; Lysosomes; Microscopy, Electron; Mitochondria; Osteoblasts; Osteosarcoma; Ribosomes