glycogen and Dwarfism--Pituitary

Currently there is no good hepatocyte model for studying growth hormone (GH) function that reflects its normal physiological roles. Here we report the establishment of a functional hepatocyte cell line, SDRL-1, from the liver of young male spontaneous dwarf rats (SDR), with isolated GH deficiency. This line has been maintained in Dulbecco's Modified Eagle Medium (DMEM)/F12 medium supplemented with 10% fetal bovine serum (FBS) with retention of a near diploid karyotype for extended periods of time. When grown as a monolayer sheet, it displayed a pavement-like appearance and contact inhibition. These cells have a poorly developed rough endoplasmic reticulum (r-ER), few mitochondria and glycogen granules, and produce a small amount of albumin and α-fetoprotein, that is enhanced when grown on a collagen gel sponge. Human recombinant GH stimulated JAK2 and STAT5b tyrosine phosphorylation and IGF-I production in a concentration-dependent manner. When the cells were cultured with GH-supplemented medium, the number of mitochondria and glycogen granules increased together with the r-ER and Golgi apparatus. A number of microvilli were observed on the surface of the cultured cells, further suggesting that this cell line is composed of normally functioning hepatocytes. In summary, we established a novel hepatocyte cell line (SDRL-1), that appears to display normal function, which we propose can serve as a good in vitro model for studying GH-target organ interactions.

Topics: Albumins; alpha-Fetoproteins; Animals; Cell Line; Dose-Response Relationship, Drug; Dwarfism, Pituitary; Endoplasmic Reticulum, Rough; Glucose; Glycogen; Golgi Apparatus; Growth Hormone; Hepatocytes; Insulin-Like Growth Factor I; Janus Kinase 2; Mitochondria; Phosphorylation; Rats; Rats, Sprague-Dawley; Receptors, Somatotropin; STAT5 Transcription Factor; Urea

Topics: Animals; Basal Metabolism; Dwarfism, Pituitary; Epinephrine; Glycogen; Heart; Hypophysectomy; Methods; Mice; Myocardium; Oxygen Consumption; Thyroidectomy

Topics: Adenosine Triphosphatases; Adolescent; Cartilage; Child; Dwarfism, Pituitary; Esterases; Female; Galactosidases; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Leucyl Aminopeptidase; Male; Nucleotidases; Oxidoreductases; Phosphoric Monoester Hydrolases; Proteins; RNA; Tibia

Topics: Adipose Tissue; Animals; Calcium; Carbohydrate Metabolism; Carbon Dioxide; Diabetes Mellitus; Diabetes Mellitus, Type 1; Dwarfism; Dwarfism, Pituitary; Fatty Acids; Glycerides; Glycogen; Growth Hormone; Hypopituitarism; Kidney Function Tests; Leucine; Lipid Metabolism; Metabolism; Pharmacology; Primates; Prolactin; Research; Sheep