glycogen and Chondrosarcoma

A lesion in the left humeral head of a 15-year-old Japanese boy was light-microscopically diagnosed as clear cell chondrosarcoma. Fresh tissues from the lesion were studied ultrastructurally. In most tumor cells, that is, clear cells, paucity of organelles, sparse free ribosomes, and low protein content in cytosol were most striking. Tumor cells had many microvilli characteristic of cartilage cells. Among scanty organelles, vesicles or cisternae of dilated rough endoplasmic reticulum were rather conspicuous, and well-developed Golgi complexes were sparse. Intercellular matrix was scanty. These results suggest that intercellular matrix substance may be synthesized substantially by rough endoplasmic reticulum, whereas the synthesized substance perhaps cannot be transported smoothly into extracellular spaces because of scanty well-developed Golgi complexes.

Topics: Adolescent; Bone Neoplasms; Cell Division; Chondrosarcoma; Glycogen; Golgi Apparatus; Humans; Humerus; Male; Microscopy, Electron

Topics: Adolescent; Adult; Aged; Bone Neoplasms; Chondrosarcoma; Collagen; Cytoskeleton; Glycogen; Humans; Microscopy, Electron; Middle Aged

A morphologic and electron microscopic study was made of two chordoid sarcomas. These lesions were compared with two classical chondrosarcomas and two chordomas. These chondrosarcoma cells showed many features common to chondrocytes, such as abundant RER, well-developed Golgi complexes, and microvillous cytoplasmatic membranes. The chordoid sarcomas bore a close morphologic resemblance to the chordomas but the ultrastructural features revealed a close relationship to the chondrosarcomas. The chordoid sarcoma and chondrosarcoma cells had scalloped cytoplasmatic membranes, variable amounts of glycogen, round or oval nuclei and microfibrils, collagen, and electron-dense granules in the ground substance. The chordoma was characterized by the presence of stellate and physalipherous cells, as well as many transitional cells, with varying nuclear morphology; dilated and irregular RER in contact with mitochondria and morphologically varied vacuoles are the main features in the cytoplasm. This study suggests that chordoid sarcoma represents a variety of the chondrosarcoma rather than a form of chordoma. These findings also support the suggestion of Weiss that chordoid sarcoma is an extraskeletal myxoid chondrosarcoma.

Topics: Cell Nucleus; Chondrosarcoma; Chordoma; Collagen; Cytoplasmic Granules; Cytoskeleton; Endoplasmic Reticulum; Glycogen; Golgi Apparatus; Humans; Intracellular Membranes; Microscopy, Electron; Vacuoles

Topics: Adult; Chondrosarcoma; Femoral Neoplasms; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Male; Microscopy, Electron; Vacuoles

Five cases of clear-cell variant of chondrosarcoma (Unni et al.) are reported. The tumors occurred in the epiphyseal region of long bones; three in the femoral head. Roentgenographically, the lesion was usually a well-defined and benign appearing one, either purely lytic (3 cases) or with central radiodensity (2 cases). Histologically, all five cases had areas of conventional chondrosarcoma; however, the greater portion of the tumor was made up of sheets of clear-cells intermixed with nonneoplastic bone trabeculae but devoid of chondroid matrix. Electron microscopic studies showed that these clear-cells possess cytoplasmic microvilli, abundant glycogen particles and prominent golgi complexes, like normal or tumorous chondroid cells usually have. In our experience, the best treatment seemed to be en bloc resection with joint replacement; indeed, despite the fact that they are true chondrosarcomas, these tumors usually have a very slow rate of growth.

Topics: Adolescent; Adult; Bone Neoplasms; Chondrosarcoma; Cytoplasm; Epiphyses; Female; Femur Head; Glycogen; Humans; Male; Microscopy, Electron; Middle Aged

The authors report five cases treated by wide resection. Pathological studies were made of the whole of the lesion. Three tumours were situated at the upper end of the femur, one at the upper end of the humerus and one at the level of the tibial plateau. The specific features were localisation in an epiphysis and histological areas of clear cells whose cytoplasm contained glycogen. From the anatomical and radiological points of view, these tumours were like chondroblastomata but their progress was more like that of chondrosarcomata. The author concludes that they should be distinguished from both types of tumour.

Topics: Adult; Bone Neoplasms; Chondroblastoma; Chondrosarcoma; Diagnosis, Differential; Epiphyses; Female; Femoral Neoplasms; Glycogen; Humans; Humerus; Male; Microscopy, Electron; Middle Aged; Osteogenesis; Radiography; Tibia

Cytologic and cytochemical examination of eighteen cases of round-cell sarcoma of bone allowed classification of these tumors into four cytologic groups. Additional cytochemical examinations based on the PAS and D-PAS reactions, and the demonstration of the activity of peroxidase, naphtol-ASD-Chloracetate esterase, alpha-naphthylacetate esterase, naphthol-AS-acetate esterase with and without sodium fluoride inhibition, acid and alkaline phosphatases yielded no evidence of uniform behavior among the individual groups or within any single group. The studies showed that a positive glycogen reaction cannot be used as a basic criterion for the classification of such tumors as Ewing's sarcoma and for regarding them as a uniform tumor group. It is possible that a pool of tumors is involved, including tumors of monocytic and probably of lymphocytic origin, reticulum-cell sarcoma, tumors of myelocytic and erythroplastic origin, stem-cell tumors, and endothelial-cell tumors. Histologic examination alone is not sufficient for the classification of round-cell sarcomas of bone, and it should be supplemented by cytologic and cytochemical or histochemical methods. Osteosarcomas (23 cases) and chondrosarcomas (8 cases) display cells which are characteristic for these tumors and which could be correlated with their benign counterparts, osteoblasts and chondroid cells. The histologically recognizable degree of malignancy of chondrosarcoma can be evaluated better with the cytologic than with the histologic technic. Indications of the possibilities of differential diagnosis based on the cytologic pictures of benign and malignant osteoplastic and chondroplastic tumors, giant-cell tumors and chordoma are discussed.

Topics: Adolescent; Bone Neoplasms; Cell Nucleolus; Cell Nucleus; Child; Chondroblastoma; Chondroma; Chondrosarcoma; Chordoma; Cytoplasm; Female; Giant Cell Tumors; Glycogen; Humans; Infant; Male; Naphthol AS D Esterase; Osteosarcoma; Periodic Acid-Schiff Reaction; Phosphoric Monoester Hydrolases; Sarcoma; Sarcoma, Ewing

Topics: Adult; Aged; Bone Neoplasms; Cartilage, Articular; Cell Nucleolus; Cell Nucleus; Chondrosarcoma; Endoplasmic Reticulum; Female; Femoral Neoplasms; Glycogen; Golgi Apparatus; Humans; Humerus; Inclusion Bodies; Male; Microscopy, Electron; Middle Aged; Mitochondria; Scapula; Sternum; Thoracic Neoplasms

Topics: Adult; Aged; Cell Nucleolus; Cell Nucleus; Chondrosarcoma; Chromatin; Cytoplasm; Endoplasmic Reticulum; Female; Femoral Neoplasms; Glycogen; Humans; Humerus; Laryngeal Neoplasms; Lipids; Male; Microscopy, Electron; Middle Aged; Prognosis; Sacrum; Tibia

Topics: Adolescent; Chondrosarcoma; Cytoplasm; Endoplasmic Reticulum; Glycogen; Golgi Apparatus; Humans; Inclusion Bodies; Leg; Male; Microscopy, Electron; Radiography

Topics: Alkaline Phosphatase; Biopsy; Bone Cysts; Bone Diseases; Bone Neoplasms; Chondroma; Chondrosarcoma; Clinical Enzyme Tests; Diagnosis, Differential; Femoral Neoplasms; Fibrosarcoma; Giant Cell Tumors; Glycogen; Hemangioma; Hemangiosarcoma; Histocytochemistry; Humans; Ilium; Lymphoma, Large B-Cell, Diffuse; Multiple Myeloma; Neoplasm Metastasis; Osteoma; Osteoma, Osteoid; Osteomyelitis; Osteosarcoma; Ribs; Sarcoma, Ewing; Tibia; Trephining; Tuberculosis, Osteoarticular

Topics: Adipose Tissue; Cartilage; Chondrosarcoma; Chordoma; Connective Tissue; Female; Fibroma; Fibrosarcoma; Glycogen; Hemangioma; Humans; Leiomyoma; Leiomyosarcoma; Lipoma; Liposarcoma; Muscle, Smooth; Neoplasms, Connective Tissue; Neoplasms, Muscle Tissue; Neoplasms, Nerve Tissue; Neurilemmoma; Neurofibroma; Sarcoma; Sarcoma, Synovial; Synovial Membrane; Uterus

Topics: Bone Diseases; Bone Neoplasms; Chondroma; Chondrosarcoma; Glycogen; Humans; Osteoma; Osteosarcoma