glycogen and Carcinoma-in-Situ

Topics: Carcinoma in Situ; Coloring Agents; Glycogen; Humans; Iodides; Male; Neoplasm Proteins; Seminoma; Staining and Labeling; Testicular Neoplasms

Topics: alpha-Fetoproteins; Amines; Animals; Bombesin; Bronchi; Calcitonin; Calmodulin; Carcinoembryonic Antigen; Carcinoma in Situ; Carcinoma, Bronchogenic; Chorionic Gonadotropin; Cytoskeletal Proteins; Esophageal Neoplasms; Glycogen; Growth Hormone; Hormones; Humans; Intestinal Neoplasms; Lung Neoplasms; Parathyroid Hormone; Physalaemin; Placental Lactogen; Precancerous Conditions; Somatostatin; Vasoactive Intestinal Peptide

Micropapillary variant of transitional cell carcinoma (TCC) is a rare entity, having a distinct micropapillary component mimicking papillary serous carcinoma of the ovary and has been reported exclusively in the urinary bladder. We experienced a case of micropapillary variant of TCC in the ureter. The tumor showed a predominant proportion of micropapillary component and accompanied a TCC in situ lesion and a high-grade TCC. A metastatic lesion in the regional lymph node also showed an entirely micropapillary pattern. Initially, our case was confused with adenocarcinoma, especially metastatic, because the micropapillary architecture resembled an abortive glandular structure and tumor cell nests were predominantly located in empty spaces mimicking vascular invasion. The patient died with peritoneal metastases 20 months after the initial diagnosis. We report the first case of a micropapillary variant of TCC occurring in the ureter.

Topics: Aged; Carcinoma in Situ; Carcinoma, Papillary; Carcinoma, Transitional Cell; Cytoplasm; Desmosomes; Diagnosis, Differential; Fatal Outcome; Glycogen; Humans; Lymph Nodes; Lymphatic Metastasis; Male; Peritoneal Neoplasms; Ureteral Neoplasms

To analyze the relationship between Lugol unstained areas and their histologic features, we applied the Lugol test to 24 specimens of resected esophagus. The staining patterns were graded into four types: grade I, hyperstaining; grade II, normal greenish brown staining; grade III, less intense staining; and grade IV, unstained. Most of the grade IV lesions were invasive carcinomas, carcinomas in situ, or severe dysplasia. The carcinomas in situ and the intraepithelial extension of the carcinomas, which were difficult to detect, were clearly shown as grade IV. On the other hand, moderate to mild dysplasia or atrophy showed grade III staining. Grade IV lesions showed well-demarcated sharp margins, whereas grade III lesions showed ill-demarcated dull margins. The grade III carcinomas, however, by the Lugol test, showed well-demarcated margins. Histologic evaluation disclosed that the staining intensity reflected well the thickness of the glycogen-containing cell layer in the lesion. The sharpness of the margin reflected the abrupt or gradual change from the glycogen-containing to non-containing cell layers. These findings suggest 1) the usefulness of the staining pattern of the Lugol test for the diagnosis of esophageal lesions such as squamous cell carcinoma and severe dysplasia, and 2) the usefulness of the Lugol test for precise delineation of the proximal resection line during surgery of esophageal carcinomas with unexpected wide extension.

Topics: Atrophy; Carcinoma in Situ; Carcinoma, Squamous Cell; Esophageal Diseases; Esophageal Neoplasms; Esophagitis; Esophagus; Glycogen; Humans; Iodides; Staining and Labeling

One hundred eight patients were studied who had anogenital lesions showing microscopic features as seen in bowenoid papulosis (BP), a recently described condition occurring most commonly in young adults. Patients typically show multiple papules, small nodules, or plaques that clinically mimic verrucae or nevocellular nevi. Although the lesions show microscopic cytologic atypia, a distinction from Bowen's disease, erythroplasia of Queyrat, and other forms of carcinoma in situ can usually be made on the basis of histologic and clinical criteria. The disorder responds to conservative treatment, although recurrences are not uncommon. Evolution of the lesions to invasive carcinoma was not observed. Mounting evidence links the development of BP to infection with human papilloma virus, but other viruses, as well as hormonal and immunologic factors, may also play a role.

Topics: Adolescent; Adult; Age Factors; Antigens, Viral; Bowen's Disease; Carcinoma in Situ; Carcinoma, Squamous Cell; DNA, Viral; Erythroplasia; Female; Glycogen; Histocytochemistry; Humans; Hyaluronic Acid; Male; Microscopy, Electron; Middle Aged; Papillomaviridae; Penile Neoplasms; Pregnancy; Sex Factors; Skin Neoplasms; Vulvar Neoplasms

The activity of the following enzymes was studied in normal, precancerous, and malignant biopsies from the human cervix uteri: hexokinase (HK), phosphofructokinase (PFK), pyruvate-kinase (PK), lactate dehydrogenase (LDH), and glucose-6-phosphate dehydrogenase (G-6-PDH). In precancerous conditions, i.e., dysplasia and carcinoma in situ without any signs of invasive carcinoma, only PK showed moderate but significant activity increases. A rise in enzyme activity in biopsies histologically classified as carcinoma in situ was found to signal the presence of invasive carcinoma in other parts of the cervix. In invasive carcinomas of the cervix, all the enzymes studied showed a two- to four-fold increase (p less than 0.01) as compared to the normal cervix. The present study failed to reveal significant differences between enzyme activities in biopsies from patients in Stage I, II, and III; no correlation could be established between enzyme activity and prognosis.

Topics: Alcohol Oxidoreductases; Biopsy; Carcinoma in Situ; Cervix Uteri; Female; Glucosephosphate Dehydrogenase; Glycogen; Hexokinase; Humans; L-Lactate Dehydrogenase; Phosphofructokinase-1; Phosphotransferases; Precancerous Conditions; Prognosis; Pyruvate Kinase; Uterine Cervical Neoplasms

Topics: Adenocarcinoma; Adenofibroma; Adult; Aged; Breast Neoplasms; Carcinoma in Situ; Carcinoma, Intraductal, Noninfiltrating; Dihydrolipoamide Dehydrogenase; Female; Glucosephosphate Dehydrogenase; Glutamate Dehydrogenase; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Lymphatic Metastasis; Mammography; Middle Aged; RNA, Neoplasm; Succinate Dehydrogenase

The stratified squamous epithelium in eleven cervical cone biopsies was studied for localization of glycogen, glycogen synthetase, branching enzyme and amylophosphorylase in areas showing normal, dysplastic epithelium and carcinoma in situ. In the normal cervix glycogen was found to be present in the intermediate and superficial cells, amylophosphorylase activity was present in the basal and parabasal layers and the branching enzyme was found largely in the lower and middle zone of the intermediate layer. As the degree of abnormality increased, a progressive decrease of glycogen and branching enzyme and increasing amylophosphorylase was found, suggesting that amylophosphorylase could be a constitutive enzyme in normal, dysplastic and neoplastic cells. The method for localization of glycogen synthetase activity was not sufficiently reproducible nor sensitive for conclusions to be drawn.

Topics: 1,4-alpha-Glucan Branching Enzyme; Carcinoma in Situ; Cervix Uteri; Epithelial Cells; Epithelium; Female; Glycogen; Glycogen Synthase; Humans; Phosphorylases; Uterine Cervical Diseases; Uterine Cervical Neoplasms

The glycogen metabolism of the human uterine cervix has been investigated in tissue specimens from 147 women with gynaecological diseases: 64 with cervical carcinomas, 6 with carcinoma in situ and 77 with nonmalignant cervical diseases. The glycogen content of the normal uterine cervix was found to be fairly constant and apparently not regulated by steroid hormones. The low content of glycogen in malgnant cervical tumours was confirmed by the present investigation. The percent investigation also showed that this abnormality was not only characteristic of invasive carcinoma but also of carcinoma in situ. A particularly low glycogen content was found in tissue samples from patients with relapse. Concerning the enzyme involved in glycogen metabolism, a significantly high activity of glycogen synthetase was found in malignant cervical biopsies, while the activity of glycogen phosphorylase did not differ significantly when comparing normal to malignant tissue.

Topics: Adult; Carcinoma; Carcinoma in Situ; Cervix Uteri; Female; Glycogen; Glycogen Synthase; Humans; Middle Aged; Phosphorylases; Uterine Cervical Neoplasms

Topics: Carcinoma; Carcinoma in Situ; Carcinoma, Squamous Cell; Female; Glycogen; Humans; Uterine Cervical Neoplasms

Topics: Carcinoma in Situ; Cervix Uteri; Female; Glycogen; Humans; Hysterectomy; Neoplasms; Uterus

Topics: Carcinoma in Situ; Cervix Uteri; Female; Glycogen; Humans; Neoplasms; Uterine Cervical Neoplasms