glycogen and Autolysis

Topics: Animals; Autolysis; Calcium; Calcium Radioisotopes; Coronary Vessels; Glycogen; Ischemia; Mitochondria, Muscle; Mitochondrial Swelling; Myocardium; Myofibrils; Papillary Muscles; Perfusion; Sarcoplasmic Reticulum; Time Factors

The progressive changes in pH, lactate content, and light and electron microscopic appearances were studied in dog myocardium undergoing a 6-hour period of autolysis in vitro at 37 degrees C. and at room temperature. At 37 degrees C. there was a rapid cumulative fall in pH during the 1st hour after excision of the heart and a corresponding increase in lactate content, but little additional change in either subsequently. The nature and sequence of the morphologic alterations at this temperature were generally similar to those which occur in ischemic myocardium in vivo. At room temperature, a much slower cumulative decrease in pH and increase in lactate content took place throughout the whole period of investigation and was paralleled by a slower rate of development of morphologic change.

Topics: Animals; Autolysis; Dogs; Glycogen; Hydrogen-Ion Concentration; Lactates; Mitochondria; Myocardial Infarction; Myocardium; Myofibrils

To define the progression of ultrastructural changes in normal muscle at post mortem, rat gastrocnemius muscles were studied at various times after storage at +4 degrees C and +22 degrees C. Degeneration of the I-zone (discoid necrosis) and membranous bodies were found to be similar to that seen in muscle diseases, and lamellar formations were seen in mitochondria. At +4 degrees C there was contraction of the sarcomere which vanished in 12 h and inter-filamentous oedema appeared. Z-line degeneration was seen at 24 h and at 4 days all Z-lines had disappeared, and the H-zone showed darkening. In the same samples collapse or ruptures of the I band were seen. At 8 days the H-zones and M-lines were still discernible. In the early stages the mitochondria showed swelling and loss of matrix granules, while later they showed broken cristae and outer membranes, and flocculent densities. At 4 days rearrangement of the cristal material into long pentalaminar "needles" was seen in a few mitochondria. At 4 and 8 days membranous bodies were seen and the T-system and sarcoplasmic reticulum showed ruptures and disintegration into vesicles. The nucleus showed increasing condensation of chromatin at the periphery and clearing of the center. Polysomes and glycogen were reduced at 2 h, and has practically vanished at 24 h. At 22 degrees C the changes were the same but appeared about 4 times as quickly as at +4 degrees C.

Topics: Animals; Autolysis; Glycogen; Mitochondria, Muscle; Mitochondrial Swelling; Muscles; Myofibrils; Rats; Sarcoplasmic Reticulum; Temperature; Time Factors

Topics: Animals; Autolysis; Glycogen; Histocytochemistry; Mitochondria, Muscle; Myocardium; Oxidation-Reduction; Rats; Sarcoplasmic Reticulum; Temperature

Topics: Adolescent; Adult; Aged; Asthma; Autolysis; Female; Glycogen; Granulocytes; Humans; Leukocytes; Male; Middle Aged

Topics: Animals; Autolysis; Dihydrolipoamide Dehydrogenase; Glutamate Dehydrogenase; Glycerolphosphate Dehydrogenase; Glycogen; Histocytochemistry; Humans; Hydrogen-Ion Concentration; Hydroxybutyrates; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Malate Dehydrogenase; Microscopy, Fluorescence; Myocardial Infarction; Myocardium; Oxidoreductases; Succinate Dehydrogenase; Swine; Time Factors

Topics: Animals; Autolysis; Formaldehyde; Glycogen; Histological Techniques; Microscopy, Electron; Mitochondria, Muscle; Mitochondrial Swelling; Myocardium; Myofibrils; Postmortem Changes; Rats; Sarcolemma; Sarcoplasmic Reticulum; Time Factors

Interactions between concanavalin A and cell wall digests of Bacillus subtilis 168 resulted in insoluble complexes as observed by double gel diffusion, turbidity, and analysis of the precipitate. The macromolecular constituent of the cell walls complexing with concanavalin A was the polyglucosylglycerol phosphate teichoic acid. The complex exhibited two pH optima: 3.1 and 7.4. The complex could be dissociated by saccharides which bind to concanavalin A. In contrast to concanavalin A-neutral polysaccharide complexes, formation of the concanavalin A-wall complex was inhibited by salts. It was subsequently shown that salts induce conformational changes in cell wall digests. The data suggested that for complex formation to occur a rigid rod conformation in the glucosylated teichoic acid is probably necessary. Concanavalin A can be used as a probe to study structural features of bacterial cell walls.

Topics: Autolysis; Bacillus subtilis; Binding Sites; Cell Wall; Chemical Precipitation; Concanavalin A; Culture Media; Densitometry; Glycogen; Hydrogen-Ion Concentration; Immunodiffusion; Iodides; Lectins; Muramidase; Potassium Chloride; Sodium; Sodium Chloride; Solubility; Teichoic Acids; Trichloroacetic Acid; Ultrasonics; Vibration; Viscosity

Topics: Animals; Autolysis; Cell Nucleus; Chromatin; Cytoplasmic Granules; Death; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Glycogen; Hydrolases; Hydrolysis; Liver; Lysosomes; Male; Microscopy, Electron; Mitochondria, Liver; Ornithine Carbamoyltransferase; Rats; Ribonucleases; Ribosomes; RNA; RNA, Ribosomal; Time Factors

Topics: Amylases; Animals; Autolysis; Cell Survival; Cell Wall; Culture Media; Dental Caries; Fructose; Gingiva; Glucose; Glycogen; Humans; Immunodiffusion; Infrared Rays; Iodine; Lactates; Lactobacillus; Lactose; Liver Glycogen; Maltose; Mutation; Polysaccharides, Bacterial; Rabbits; Spectrophotometry; Streptococcus

Topics: Animals; Autolysis; Glycogen; Humans; Infant; Infant, Newborn; Liver Glycogen; Myocardium; Postmortem Changes

Topics: Animals; Autolysis; Cell Nucleus; Glycogen; Microscopy, Electron; Mitochondria, Muscle; Myocardium; Myofibrils; Postmortem Changes; Rats; Time Factors

Topics: Acridines; Animals; Autolysis; Autopsy; Cardiomegaly; Clinical Enzyme Tests; Coronary Disease; Death, Sudden; Diagnosis, Differential; Forensic Medicine; Glycogen; Humans; Hydrogen-Ion Concentration; Microscopy, Fluorescence; Myocardial Infarction; Myocardium; Oxidoreductases; Staining and Labeling; Swine

Topics: Animals; Autolysis; Dogs; Glycogen; Ischemia; Lactates; Microscopy, Electron; Mitochondria, Muscle; Myocardium; Oxygen Consumption; Pyruvates; Succinates

Topics: Autolysis; Biological Transport, Active; Epithelium; Female; Fetus; Glycogen; Hair; Humans; Keratins; Morphogenesis; Phagocytosis; Pregnancy; Sebaceous Glands