glyceryl-behenate and Mouth-Neoplasms

Oral squamous cellular carcinoma (OSCC) is considered a life-threatening disease with detection in late stages, which forces us to opt for dangerous treatment with a combination of chemotherapy and radiotherapy. Herbal components such as piperine and quercetin are derived from edible sources, proving their anticancer potential against oral cancer cells in vitro. Encapsulation into lipid matrix-mediated nanostructured lipid carriers (NLCs) can make both drugs bio-accessible. NLCs were synthesised using the high shear homogenisation method and characterised for their physicochemical properties, followed by in vitro cellular evaluation in FaDu oral cancer cells. NLCs showed negatively charged particles smaller than 180 nm with a polydispersity index (PDI) of <0.3. Both drugs were found to encapsulate sufficiently, with >85% entrapment efficiency and an improved drug release profile compared to their pristine counterparts. Differential scanning calorimetry (DSC) thermograms showed conversion into an amorphous matrix in lyophilized NLCs, which was supported by X-ray diffraction (XRD) analysis. The cytotoxicity assay showed the IC

Topics: Alkaloids; Animals; Apoptosis; Benzodioxoles; Drug Liberation; Drug Screening Assays, Antitumor; Fatty Acids; Humans; Membrane Potential, Mitochondrial; Mouth Neoplasms; Nanoparticle Drug Delivery System; Nanostructures; Particle Size; Piperidines; Polyunsaturated Alkamides; Quercetin; Rats; Squamous Cell Carcinoma of Head and Neck; Tissue Distribution