ginsenoside-m1 and Osteoarthritis

Osteoarthritis (OA) is a common joint disease, and studies have reported that the endoplasmic reticulum stress (ERS) in chondrocytes caused by the cartilage tissue damage could mediate the activation of Nod-like receptor protein 3 (NLRP3) inflammasomes through inositol-requiring enzyme 1 alpha (IRE1α) and thioredoxin interacting protein (TXNIP). Ginsenoside compound K (CK) has an inhibitory effect on IRE1α activation. However, the role of IRE1α-TXNIP and its interaction with CK are still unclear. In this study, we examined the role and mechanism of action of CK in OA. We found that CK ameliorated OA and ERS in IL-1β-treated chondrocytes and a monoiodoacetate-induced rat OA model. The effect of CK on inflammation, pyroptosis, and ERS was blocked by the ERS inducer tunicamycin. In conclusion, CK hindered OA progression by inhibiting the ERS-IRE1α-TXNIP-NLRP3 axis. Overall, our data indicate that CK could be useful in the treatment of OA and other chronic inflammatory diseases.

Topics: Animals; Apoptosis; Cell Cycle Proteins; Chondrocytes; Endoplasmic Reticulum Stress; Endoribonucleases; Inflammasomes; NLR Family, Pyrin Domain-Containing 3 Protein; NLR Proteins; Osteoarthritis; Protein Serine-Threonine Kinases; Pyroptosis; Rats

Ginsenosides have been used traditionally as an oriental medicine. However, the anti-osteoarthritic effect of ginsenoside compound K (hereafter referred to as CK) has not been reported. Therefore, in this study, the protective effects of CK were evaluated in silico and in vitro using H

Topics: Alkaline Phosphatase; Animals; Calcification, Physiologic; Cell Line; Cell Survival; Collagen; Computer Simulation; Core Binding Factor Alpha 1 Subunit; Drug Evaluation, Preclinical; Ginsenosides; Hydrogen Peroxide; I-kappa B Kinase; Interleukin-1beta; Mice, Inbred C57BL; Molecular Docking Simulation; Nitric Oxide; Osteoarthritis; Osteoblasts; Osteocalcin; Protein Kinase Inhibitors; RNA, Messenger