gingerol and Osteoarthritis

Protandim and 6-gingerol, two potent nutraceuticals, have been shown to decrease free radicals production through enhancing endogenous antioxidant enzymes. In this study, we evaluated the effects of these products on the expression of different factors involved in osteoarthritis (OA) process. Human OA chondrocytes were treated with 1 ng/ml IL-1β in the presence or absence of protandim (0-10 μg/ml) or 6-gingerol (0-10 μM). OA was induced surgically in mice by destabilization of the medial meniscus (DMM). The animals were treated weekly with an intraarticular injection of 10 μl of vehicle or protandim (10 μg/ml) for 8 weeks. Sham-operated mice served as controls. In vitro, we demonstrated that protandim and 6-gingerol preserve cell viability and mitochondrial metabolism and prevented 4-hydroxynonenal (HNE)-induced cell mortality. They activated Nrf2 transcription factor, abolished IL-1β-induced NO, PGE

Topics: Animals; Anti-Inflammatory Agents; Apoptosis; Catechols; Cell Survival; Cells, Cultured; Chondrocytes; Dietary Supplements; Disease Models, Animal; Drugs, Chinese Herbal; Fatty Alcohols; Glutathione Transferase; Humans; Injections, Intra-Articular; Interleukin-1beta; Mice; NF-E2-Related Factor 2; Osteoarthritis; Signal Transduction