gilvocarcin-v and Xeroderma-Pigmentosum

gilvocarcin-v has been researched along with Xeroderma-Pigmentosum* in 1 studies

Other Studies

1 other study(ies) available for gilvocarcin-v and Xeroderma-Pigmentosum

Article	Year
Photoactivated gilvocarcin V induces DNA-protein crosslinking in genes for human ribosomal RNA and dihydrofolate reductase. Photochemistry and photobiology, 1994, Volume: 60, Issue:3 The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose-dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA-protein crosslinking. Topics: Aminoglycosides; Anti-Bacterial Agents; Blotting, Southern; Cell Line; Cell Survival; Coumarins; DNA; DNA-Binding Proteins; DNA, Ribosomal; Fanconi Anemia; Fibroblasts; Genes; Glycosides; Humans; Intercalating Agents; Methoxsalen; Photochemistry; Photosensitizing Agents; RNA, Ribosomal; Tetrahydrofolate Dehydrogenase; Xeroderma Pigmentosum	1994

Article

Year

Photoactivated gilvocarcin V induces DNA-protein crosslinking in genes for human ribosomal RNA and dihydrofolate reductase.

Photochemistry and photobiology, 1994, Volume: 60, Issue:3

The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose-dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA-protein crosslinking.

Topics: Aminoglycosides; Anti-Bacterial Agents; Blotting, Southern; Cell Line; Cell Survival; Coumarins; DNA; DNA-Binding Proteins; DNA, Ribosomal; Fanconi Anemia; Fibroblasts; Genes; Glycosides; Humans; Intercalating Agents; Methoxsalen; Photochemistry; Photosensitizing Agents; RNA, Ribosomal; Tetrahydrofolate Dehydrogenase; Xeroderma Pigmentosum

1994