germacrone and Acute-Kidney-Injury

Cisplatin is a widely used chemotherapy for solid tumors; however, its benefits are limited by serious nephrotoxicity, particularly in proximal tubular cells. The present study investigated the renoprotective effect and mechanisms of germacrone, a bioactive terpenoid compound found in Curcuma species on cisplatin-induced toxicity of renal cells. Germacrone (50 and 100 µM) attenuated apoptosis of human renal proximal tubular cells, RPTEC/TERT1 following treatment with 50 µM cisplatin and for 48 h. Co-treating RPTEC/TERT1 cells with cisplatin and germacrone significantly reduced cellular platinum content compared with cisplatin treatment alone. The effect of germacrone on organic cation transporter 2 (OCT2) which is a transporter responsible for cisplatin uptake was determined. Germacrone showed an inhibitory effect on OCT2-mediated methyl-4-phenylpyridinium acetate (

Topics: Acute Kidney Injury; Animals; CHO Cells; Cisplatin; Cricetulus; Drug Evaluation, Preclinical; Epithelial Cells; Hep G2 Cells; Humans; Inhibitory Concentration 50; Kidney Tubules, Proximal; Octamer Transcription Factor-1; Organic Cation Transporter 2; Sesquiterpenes, Germacrane