geoditin-a and Colonic-Neoplasms

Geoditin A, an isomalabaricane triterpene isolated from the marine sponge Geodia japonica, has been demonstrated to dissipate mitochondrial membrane potential, activate caspase 3, decrease cytoplasmic proliferating cell nuclear antigen (PCNA), and induce apoptosis of leukemia cells, but the underlying mechanism remains unclear [1]. In this study, we found fragmentation of Golgi structure, suppression of transferrin receptor expression, production of oxidants, and DNA fragmentation in human colon cancer HT29 cells after treatment with geoditin A for 24 h. This apoptosis was not abrogated by chelation of intracellular iron with salicylaldehyde isonicotinoyl hydrazone (SIH), but suppressed by N-acetylcysteine (NAC), a thiol antioxidant and GSH precursor, indicating that the cytotoxic effect of geoditin A is likely mediated by a NAC-inhibitable oxidative stress. Our results provide a better understanding of the apoptotic properties and chemotherapeutical potential of this marine triterpene.

Topics: Acetylcysteine; Adenocarcinoma; Animals; Antineoplastic Agents; Apoptosis; Cell Proliferation; Colonic Neoplasms; DNA Fragmentation; Dose-Response Relationship, Drug; Down-Regulation; Endocytosis; Free Radical Scavengers; Golgi Apparatus; HT29 Cells; Humans; Inhibitory Concentration 50; Iron Chelating Agents; Marine Toxins; Oxidants; Oxidative Stress; Receptors, Transferrin; Resorcinols; Triterpenes