gastrins and Splenic-Diseases

To study the underlying mechanism of acupuncture of "Zusanli" (ST 36) in the treatment of spleen deficiency syndrome.. Twenty-six adult Wistar rats were randomly divided into normal control group (n = 6), model group (n = 8), saline group (n = 6) and acupuncture group (n = 6). Spleen deficiency syndrome model was duplicated by intragastric perfusion of 50% alcohol (on the 1st day, 1 mL/100 g) and white vinegar for 9 days (1 mL/100 g). Before decapitation, the animals in all the 4 groups were forced to swim in a water pool under temperature of 18 degrees C till exhaustion (sinking to the bottom of the water pool). Blood samples were collected after decapitation of rats for detecting contents of serum gastrin and cortisol with radioimmunoassay. Unilateral "Zusanli" (ST 36) was punctured and stimulated manually then the needle was retained for 20 min. The treatment was given once daily and continuously for 10 days.. Compared with normal control group, serum gastrin and cortisol contents of model and saline groups decreased significantly (P < 0.05), while compared with model group and saline group, both gastrin and cortisol levels increased remarkably in acupuncture group (P < 0.05). No significant differences were found between saline group and model group, and between normal control group and acupuncture group in serum gastrin and cortisol levels (P > 0.05).. Acupuncture of "Zusanli" (ST 36) can suppress alcohol and vinegar induced decrease of serum gastrin and cortisol, which may contribute to its effect in the treatment of spleen deficiency syndrome in the rat.

Topics: Acupuncture Therapy; Animals; Gastrins; Hydrocortisone; Male; Medicine, Chinese Traditional; Rats; Rats, Wistar; Splenic Diseases; Syndrome

50 adult Wistar male rats were used and divided into 4 groups, i.e. normal control group, experimental Spleen Deficiency group induced by rhubarb, spontaneous recovery group and therapeutic group treated with Chinese recipe (Si Jun Zi decoction). All the animals of the 4 groups were killed simultaneously, and the jejunum and ileum were removed and processed for demonstration of gastrin cells and 5-HT cells according to immunohistochemical PAP technique. In addition, HE stained samples were prepared. The immunoreactivities of the two types of enteroendocrine cells were observed and semiquantitative estimation were performed under light microscopy. In addition, the immunoreactivities of 5-HT cells in normal control and experimental Spleen Deficiency group were measured by microspectrocytophotometer (MPV 2, Leitz). All the data were treated statistically. This study revealed that there were no obvious histological changes in the mucosa among the 4 groups. In the jejunum, the percentage of gastrin cells(+) in experimental Spleen Deficiency group was more than that of the normal control group, while the percentage of gastrin cells( ) was less than that of the normal control group. As compared with spontaneous recovery group, it showed contrary to the above result in the therapeutic group. No gastrin cells were found in the ileum in all the 4 groups. the percentage of 5-HT cells did not show significant changes in the jejunum and ileum among the 4 groups. But immuno-reactivity in the 5-HT cell was less than that of the normal control group in the jejunum of the Spleen Deficiency group.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Animals; Drugs, Chinese Herbal; Enterochromaffin Cells; Gastrins; Immunohistochemistry; Intestine, Small; Male; Plants, Medicinal; Rats; Rats, Wistar; Rheum; Serotonin; Splenic Diseases; Yang Deficiency

The character of "Spleen Deficiency" (SD) Syndrome was evaluated by determination of the blood levels of various gastroenteropancreatic hormones and thyroid hormone. Patients of SD group were diagnosed clinically by Syndrome Differentiation, and those of the normal group were volunteer blood donors. RIA was used to determine all the hormones except T3RUR.. TT3, FT3 of the SD group were very significantly lower than that of normal group (P less than 0.001). The levels of rT3 in SD group were significantly higher than normal control (P less than 0.05). For TT4, T3RUR and FT4, there were no significant differences between the two groups. Among the gastroenteropancreatic hormones, gastrin in the SD group was significantly lower than that of the normal group (P less than 0.01), motilin was very significantly higher (P less than 0.002). The value of pp was similar in the two groups, under physiological conditions, however, in adults the hormone tended to increase with age. Since the average age of patients in the SD group was 54.9, and that of the normal group was 38.5, remarkably lower values were seen in the SD group. Other hormones such as VIP, SST, glucagon and insulin were of no significant difference between the two groups. The result showed that thyroid hormone and some of gastroenteric hormones in the blood of patients with SD after major operations were quite different from normal, and was common to patients with general SD Syndrome, with low T3 syndrome.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Adult; Aged; Cholecystectomy; Female; Gastrectomy; Gastrins; Humans; Male; Middle Aged; Motilin; Postoperative Complications; Splenic Diseases; Thyroxine; Triiodothyronine; Yang Deficiency

In this paper, 89 Spleen deficiency (SD) patients' gastric mucosa specimens obtained during fibrogastroscopy mucosal biopsy were observed with immunocytochemical-stereologic-quantitative analysis method. The number of G cells counted and the content of basic gastrin in serum measured. This study found that the difference of the number of G cells and the content of gastrin in serum possessed statistical significance between two types of SD. The results suggested the hyperplasia and hypersecretion of G cells in gastric antrum might be an important pathological mechanism for different types of SD.

Topics: Adolescent; Adult; Aged; Female; Gastric Mucosa; Gastrins; Gastritis; Gastroscopy; Humans; Immunohistochemistry; Male; Medicine, Chinese Traditional; Middle Aged; Peptic Ulcer; Pyloric Antrum; Splenic Diseases

Topics: Adult; Aged; Animals; Female; Gastrins; Humans; Male; Medicine, Chinese Traditional; Mice; Middle Aged; Splenic Diseases

Topics: Gastrins; Gastrointestinal Transit; Humans; Intestinal Absorption; Medicine, Chinese Traditional; Spleen; Splenic Diseases

Topics: Gastrins; Humans; Liver Diseases, Parasitic; Radioimmunoassay; Schistosomiasis; Splenic Diseases