gastrin-i and Stomach-Neoplasms

Parietal cells play a fundamental role in stomach maintenance, not only by creating a pathogen-free environment through the production of gastric acid, but also by secreting growth factors important for homeostasis of the gastric epithelium. The gastrointestinal hormone gastrin is known to be a central regulator of both parietal cell function and gastric epithelial cell proliferation and differentiation. Our previous gene expression profiling studies of mouse stomach identified parathyroid hormone-like hormone (PTHLH) as a potential gastrin-regulated gastric growth factor. Although PTHLH is commonly overexpressed in gastric tumors, its normal expression, function, and regulation in the stomach are poorly understood. In this study we used pharmacologic and genetic mouse models as well as human gastric cancer cell lines to determine the cellular localization and regulation of this growth factor by the hormone gastrin. Analysis of

Topics: Animals; Cell Line, Tumor; Gastrins; Gene Expression Regulation, Neoplastic; Genotype; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Parathyroid Hormone-Related Protein; Parietal Cells, Gastric; Phenotype; RNA Processing, Post-Transcriptional; RNA Stability; RNA, Messenger; Stomach Neoplasms; Time Factors; Transcriptional Activation; Up-Regulation