ganglioside--gd2 and Liver-Cirrhosis

The neural ganglioside GD2 has recently been reported to be a novel surface marker that is only expressed on human bone marrow mesenchymal stem cells within normal marrow. In this study, an MRI-visible, targeted, non-viral vector for effective gene delivery to human bone marrow mesenchymal stem cells was first synthesized by attaching a targeting ligand, the GD2 single chain antibody (scAbGD2), to the distal ends of PEG-g-PEI-SPION. The targeted vector was then used to condense plasmid DNA to form nanoparticles showing stable small size, low cytotoxicity, and good biocompatibility. Based on a reporter gene assay, the transfection efficiency of targeting complex reached the highest value at 59.6% ± 4.5% in human bone marrow mesenchymal stem cells, which was higher than those obtained using nontargeting complex and lipofectamine/pDNA (17.7% ± 2.9% and 34.9% ± 3.6%, respectively) (P<0.01). Consequently, compared with the nontargeting group, more in vivo gene expression was observed in the fibrotic rat livers of the targeting group. Furthermore, the targeting capacity of scAbGD2-PEG-g-PEI-SPION was successfully verified in vitro by confocal laser scanning microscopy, Prussian blue staining, and magnetic resonance imaging. Our results indicate that scAbGD2-PEG-g-PEI-SPION is a promising MRI-visible non-viral vector for targeted gene delivery to human bone marrow mesenchymal stem cells.

Topics: Adult; Animals; Cell Survival; Cells, Cultured; Female; Gangliosides; Genetic Vectors; Green Fluorescent Proteins; Humans; Liver Cirrhosis; Magnetic Resonance Imaging; Magnetite Nanoparticles; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Microscopy, Confocal; Particle Size; Polyethylene Glycols; Polyethyleneimine; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Single-Chain Antibodies; Transfection; Transplantation, Heterologous; Young Adult