ganglio-n-triaosylceramide and Melanoma

ganglio-n-triaosylceramide has been researched along with Melanoma* in 1 studies

Other Studies

1 other study(ies) available for ganglio-n-triaosylceramide and Melanoma

ArticleYear
Optimal conditions to radiolabel (3H or 14C) aminosugar-containing glycosphingolipids by de-N-acetylation and re-N-acetylation.
    Biochimica et biophysica acta, 1995, Jun-09, Volume: 1244, Issue:2-3

    The optimal conditions were examined for selective re-N-acetylation with 14C or 3H.acetic anhydride of de-N-acetylated aminosugar-containing glycosphingolipids. Re-N-acetylation, which is nearly quantitative within 10 minutes in methanol, occurs selectively up to a maximal 100% yield when using a molar ratio of 5 mol of acetic anhydride per mole of aminosugar present in the glycosphingolipid. Above this molar ratio, it was observed some O-acetylation of carbohydrates which could be removed by mild alkali treatment. The method allows the choice of 14C- or 3H-labeling of glycosphingolipids with a final specific radioactivity which depends solely on the one of acetic anhydride. The binding of specific antibodies to glycosphingolipids, which was abolished upon de-N-acetylation, was again detectable after re-N-acetylation with radioactive acetic anhydride, suggesting that the native structures were recovered. This procedure of radiolabeling offers safety, rapidity and broad applicability to alkali-stable aminosugar-containing glycosphingolipids.

    Topics: Acetylation; Animals; Brain Chemistry; Carbon Radioisotopes; Cattle; Chromatography, High Pressure Liquid; Erythrocytes; G(M1) Ganglioside; G(M3) Ganglioside; Gangliosides; Glycosphingolipids; Humans; Isotope Labeling; Melanoma; Tritium

1995