gamma-linolenic-acid and Mesenteric-Vascular-Occlusion

The antithrombotic effect of a synthetic symmetrical triglyceride having eicosapentaenoic acid (EPA) at positions 1 and 3, and gamma-linolenic acid (GLA) at position 2 was investigated. Administration of the triglyceride significantly increased thrombus formation time and thrombotic occlusion time induced by light irradiation and a fluorescent dye in guinea pigs after 14 days administration compared to that of soybean oil. The antithrombotic effect of the triglyceride was similar to that of EPA ethyl ester. Administration of the triglyceride increased GLA, dihomo-gamma-linolenic acid (DGLA) and EPA contents in plasma and the liver, and the ratio of DGLA to arachidonic acid. These results might be responsible for this antithrombotic effect.

Topics: 8,11,14-Eicosatrienoic Acid; Animals; Arachidonic Acid; Eicosapentaenoic Acid; Fatty Acids; Fibrinolytic Agents; Fluorescein; Fluoresceins; gamma-Linolenic Acid; Guinea Pigs; Light; Lipids; Liver; Male; Mesenteric Vascular Occlusion; Mesentery; Molecular Structure; Photochemistry; Soybean Oil; Structure-Activity Relationship; Triglycerides; Venules

Diet including mold oil from a lipid accumulative fungus, containing gamma-linolenic acid, showed an inhibitory effect on thrombus formation in the microvessels of rats by the light/dye method of the authors. Male Wistar rats were fed for 3 to 4 weeks with two series of experimental diets and were examined for thrombus formation. The thrombus formation times to totally occlude, ts, were 347 sec for (mold + soybean)-oil and 236 sec for (palm + soybean)-oil in the first series of diets and 1288 sec for mold oil, 538 sec for olive oil and 575 sec for safflower oil in the second series of diets. Fatty acid composition of plasma, erythrocyte and liver lipids showed an increase in arachidonate content with the diet including the mold oil. Higher arachidonate content seem favorable in inhibiting thrombus formation with increasing PGI2 formation. In terms of the level of lipid hydroperoxides, indicated as a desaturation index of constituent fatty acids, the higher desaturation index with safflower oil gave shorter ts, which suggested some oxygen derived free radicals from polyunsaturated fatty acids were involved in the mechanism of thrombogenesis study by this method.

Topics: Animals; Blood Pressure; Dietary Fats; Fluorescein; Fluoresceins; Free Radicals; Fungi; gamma-Linolenic Acid; Light; Linolenic Acids; Lipid Peroxidation; Male; Mesenteric Vascular Occlusion; Plant Oils; Rats; Rats, Inbred Strains; Thrombosis; Weight Gain