gambogic-acid and Retinal-Neovascularization

gambogic-acid has been researched along with Retinal-Neovascularization* in 1 studies

Other Studies

1 other study(ies) available for gambogic-acid and Retinal-Neovascularization

Article	Year
Gambogic acid ameliorates diabetes-induced proliferative retinopathy through inhibition of the HIF-1α/VEGF expression via targeting PI3K/AKT pathway. Life sciences, 2018, Jan-01, Volume: 192 Gambogic acid (GA) is one of active components of Chinese medicine gamboges resin. Diabetic retinopathy (DR) is a most serious microvascular complication of diabetes and also the leading cause of blindness. The aim of this study is to evaluate the beneficial effect of GA on diabetes-induced retinal angiogenesis and further explore the potential mechanisms.. High glucose (HG)-treated RF/6A cells and STZ-induced diabetic mice were used as in vitro and in vivo models. Then the effects of GA on proliferation, migration and tube formation in RF/6A cells and pathomorphological changes in STZ-induced diabetic mice were determined. The activation of HIF-1α/VEGF and PI3K/AKT signaling pathways was assessed by various molecular biological experiments.. According to our results, GA inhibited HG-induced proliferation, migration and tube formation in choroid-retinal endothelial RF/6A cells. The upregulation of HIF-1α and VEGF induced by HG in RF/6A cells was restrained by GA treatment significantly. Moreover, GA suppressed retinal pathomorphological changes and angiogenesis in STZ-induced diabetic mice in vivo, and also inhibited the activation of HIF-1α/VEGF pathway induced by diabetics. Finally, GA suppressed the activation of PI3K/AKT signaling pathway in STZ-induced diabetic mice in vivo and in HG-induced RF/6A cells in vitro. Further activation of PI3K/AKT pathway by IGF-1 restrained the beneficial effect of GA in RF/6A cells.. Our results provide evidence that GA may ameliorate diabetes-induced retinal angiogenesis, which are proofs that GA may be developed as a potential drug for treating DR. Topics: Animals; Antineoplastic Agents, Phytogenic; Cell Movement; Cell Proliferation; Diabetes Mellitus, Experimental; Diabetic Retinopathy; Glucose; Hypoxia-Inducible Factor 1, alpha Subunit; Macaca mulatta; Male; Mice, Inbred C57BL; Oncogene Protein v-akt; Phosphoinositide-3 Kinase Inhibitors; Retinal Neovascularization; Signal Transduction; Vascular Endothelial Growth Factor A; Wound Healing; Xanthones	2018

Article

Year

Gambogic acid ameliorates diabetes-induced proliferative retinopathy through inhibition of the HIF-1α/VEGF expression via targeting PI3K/AKT pathway.

Life sciences, 2018, Jan-01, Volume: 192

Gambogic acid (GA) is one of active components of Chinese medicine gamboges resin. Diabetic retinopathy (DR) is a most serious microvascular complication of diabetes and also the leading cause of blindness. The aim of this study is to evaluate the beneficial effect of GA on diabetes-induced retinal angiogenesis and further explore the potential mechanisms.. High glucose (HG)-treated RF/6A cells and STZ-induced diabetic mice were used as in vitro and in vivo models. Then the effects of GA on proliferation, migration and tube formation in RF/6A cells and pathomorphological changes in STZ-induced diabetic mice were determined. The activation of HIF-1α/VEGF and PI3K/AKT signaling pathways was assessed by various molecular biological experiments.. According to our results, GA inhibited HG-induced proliferation, migration and tube formation in choroid-retinal endothelial RF/6A cells. The upregulation of HIF-1α and VEGF induced by HG in RF/6A cells was restrained by GA treatment significantly. Moreover, GA suppressed retinal pathomorphological changes and angiogenesis in STZ-induced diabetic mice in vivo, and also inhibited the activation of HIF-1α/VEGF pathway induced by diabetics. Finally, GA suppressed the activation of PI3K/AKT signaling pathway in STZ-induced diabetic mice in vivo and in HG-induced RF/6A cells in vitro. Further activation of PI3K/AKT pathway by IGF-1 restrained the beneficial effect of GA in RF/6A cells.. Our results provide evidence that GA may ameliorate diabetes-induced retinal angiogenesis, which are proofs that GA may be developed as a potential drug for treating DR.

Topics: Animals; Antineoplastic Agents, Phytogenic; Cell Movement; Cell Proliferation; Diabetes Mellitus, Experimental; Diabetic Retinopathy; Glucose; Hypoxia-Inducible Factor 1, alpha Subunit; Macaca mulatta; Male; Mice, Inbred C57BL; Oncogene Protein v-akt; Phosphoinositide-3 Kinase Inhibitors; Retinal Neovascularization; Signal Transduction; Vascular Endothelial Growth Factor A; Wound Healing; Xanthones

2018