galloylpaeoniflorin and Acute-Lung-Injury

This research is to study the relationship between HPLC fingerprints of Moutan Cortex, Paeoniae Radix Rubra and Paeoniae Radix Alba and their activity on lipopolysaccharide-induced acute lung injury. HPLC fingerprints of each extract of Moutan Cortex,Paeoniae Radix Rubra and Paeoniae Radix Alba were established by an optimized HPLC-MS method. The activities of all samples against protein and tumor necrosis a factor were tested by the model of lipopolysaccharide-induced acute lung injury. The possible relationship between HPLC-MS fingerprints and the activitieswere deduced by the Partial least squares regression analysis method. Samples were analyzed by HPLC-MS/MS to identify the major peaks. The results showed that each sample had some effect on acute lung injury. Four components with a lager contribution rate of efficacy were calculated by the research of spectrum-effect relationship. Moutan Cortex exhibited good activity on acute lung injury, and gallic acid, paeoniflorin, galloylpaeoniflorin and paeonol were the main effective components.

Topics: Acetophenones; Acute Lung Injury; Animals; Bridged Bicyclo Compounds, Heterocyclic; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Gallic Acid; Glucosides; Lipopolysaccharides; Male; Monoterpenes; Paeonia; Plant Roots; Rats; Rats, Wistar; Tandem Mass Spectrometry