galangin has been researched along with Thrombosis* in 1 studies
1 review(s) available for galangin and Thrombosis
Article | Year |
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Identification of Antithrombotic Natural Products Targeting the Major Substrate Binding Pocket of Protein Disulfide Isomerase.
Protein disulfide isomerase (PDI) is a vital oxidoreductase. Extracellular PDI promotes thrombus formation but does not affect physiological blood hemostasis. Inhibition of extracellular PDI has been demonstrated as a promising strategy for antithrombotic treatment. Herein, we focused on the major substrate binding site, a unique pocket in the PDI b' domain, and identified four natural products binding to PDI by combining virtual screening with tryptophan fluorescence-based assays against a customized natural product library. These hits all directly bound to the PDI-b' domain and inhibited the reductase activity of PDI. Among them, galangin showed the most prominent potency (5.9 μM) against PDI and as a broad-spectrum inhibitor for vascular thiol isomerases. Topics: Animals; Binding Sites; Biological Products; Fibrinolytic Agents; Mice; Molecular Docking Simulation; Protein Binding; Protein Disulfide-Isomerases; Thrombosis | 2022 |