galactomannan and Swine-Diseases

To assess the efficacy of a β-galactomannan oligosaccharide (ß-GMOS) for the control of Salmonella infection in fattening pigs.. Three different doses (0.5, 3 and 2 kg ß-GMOS per ton of feed) were used during the entire period of growing in three similar and independent field trials carried out in a small fattening unit (≈ 100 pigs). Treatment was randomly assigned to half of the pens. Individual serum samples (20-25 per group) were collected at different times during the fattening period and a similar number of faecal samples during the fattening period and at slaughter. In addition, mesenteric lymph nodes were collected at slaughter. Herdcheck(®) Swine Salmonella ELISA was used for serological analyses, the ISO 6579:2002/Amd 1 : 2007 for bacteriology and the PFGE for molecular characterization of Salmonella strains. The addition of ≥ 2 kg t(-1) of ß-GMOS to the pig diet during the entire fattening period was associated with a reduction in Salmonella prevalence, shedding and seroconversion.. Feed supplementation with ß-GMOS may be a useful complementary tool for the control of salmonellosis in fattening pigs.. ß-GMOS may be a complementary way of reducing Salmonella shedding and infection in fattening pigs.

Topics: Animals; Bacterial Shedding; Dietary Supplements; Galactose; Mannans; Oligosaccharides; Salmonella; Salmonella Infections, Animal; Seroepidemiologic Studies; Swine; Swine Diseases

The inhibition of the attachment of bacteria to the intestine by receptor analogues could be a novel approach to prevent enterotoxigenic Escherichia coli (ETEC) K88-induced diarrhoea in piglets. The objective of the present study was to screen the ability of different feed ingredients (FI) to bind to ETEC K88 (adhesion test, AT) and to block its attachment to the porcine intestinal mucus (blocking test, BT) using in vitro microtitration-based models. In the AT, wheat bran (WB), casein glycomacropeptide (CGMP) and exopolysaccharides exhibited the highest adhesion to ETEC K88 (P< 0·001). In the BT, WB, CGMP and locust bean (LB) reduced the number of ETEC K88 attached to the intestinal mucus (P< 0·001). For WB and LB, fractionation based on their carbohydrate components was subsequently carried out, and each fraction was evaluated individually. None of the WB fractions reduced the adhesion of ETEC K88 to the mucus as did the original extract, suggesting that a protein or glycoprotein could be involved in the recognition process. With regard to the LB fractions, the water-extractable material reduced the adhesion of ETEC K88 (P< 0·001) to the mucus similar to the original extract (P< 0·001), indicating, in this case, that galactomannans or phenolic compounds could be responsible for the recognition process. In conclusion, among the FI screened, the soluble extracts obtained from WB, LB and CGMP exhibited the highest anti-adhesive properties against ETEC K88 in the BT. These results suggest that they may be good candidates to be included in diets of weaned piglets for the prevention of ETEC K88-induced diarrhoea.

Topics: Animals; Bacterial Adhesion; Biological Products; Caseins; Diarrhea; Dietary Fiber; Enterotoxigenic Escherichia coli; Escherichia coli Infections; Fabaceae; Galactose; Intestinal Mucosa; Intestines; Mannans; Peptide Fragments; Phenols; Polysaccharides, Bacterial; Swine; Swine Diseases; Triticum