g(m3)-ganglioside and Hypercholesterolemia

g(m3)-ganglioside has been researched along with Hypercholesterolemia* in 1 studies

Other Studies

1 other study(ies) available for g(m3)-ganglioside and Hypercholesterolemia

ArticleYear
NPC1L1-dependent intestinal cholesterol absorption requires ganglioside GM3 in membrane microdomains.
    Journal of lipid research, 2018, Volume: 59, Issue:11

    Intestinal cholesterol absorption is a key regulator of systemic cholesterol homeostasis. Excessive dietary cholesterol and its intestinal uptake lead to hypercholesterolemia, a major risk factor for cardiovascular disease. Intestinal cholesterol uptake is mediated by Niemann-Pick C1-like 1 (NPC1L1), a transmembrane protein localized in membrane microdomains (lipid rafts) enriched in gangliosides and cholesterol. The roles of gangliosides, such as monosialodihexosylganglioside (GM3) and its synthesizing enzyme GM3 synthase (GM3S), in NPC1L1-dependent cholesterol uptake have not been examined previously. Here, we examined NPC1L1-dependent cholesterol uptake in a cell model as well as in wild-type and apoE-deficient mice fed normal or high-cholesterol diets. We showed that NPC1L1-dependent cholesterol uptake was impaired in GM3S-deficient cells and that GM3S deficiency promoted resistance to hypercholesterolemia in both wild-type and apoE-deficient mice fed the high-cholesterol but not the normal diet. Our findings suggest that GM3 and related gangliosides are essential for NPC1L1-mediated intestinal cholesterol absorption and are potential targets for hypercholesterolemia therapy.

    Topics: Animals; Biological Transport; Cholesterol; G(M3) Ganglioside; HEK293 Cells; Humans; Hypercholesterolemia; Immunohistochemistry; Intestinal Absorption; Lipoproteins; Male; Membrane Microdomains; Membrane Transport Proteins; Mice; Mice, Inbred C57BL; Mice, Knockout; Tandem Mass Spectrometry

2018