g(m3)-ganglioside and Calcinosis

g(m3)-ganglioside has been researched along with Calcinosis* in 1 studies

Other Studies

1 other study(ies) available for g(m3)-ganglioside and Calcinosis

Article	Year
TGF-beta 1 and 25-hydroxycholesterol stimulate osteoblast-like vascular cells to calcify. The Journal of clinical investigation, 1994, Volume: 93, Issue:5 Previous studies in our laboratory demonstrated messenger RNA for bone morphogenetic protein-2a in human calcified plaque, suggesting that arterial calcification is a regulated process, similar to osteogenesis. To further test this hypothesis, we have isolated and cloned a subpopulation of cells from bovine aortic media that show osteoblastic potential. These novel cells are primarily distinguished from smooth muscle cells by expression of a surface marker preliminarily identified as a modified form of the ganglioside sialyl-lactosylceramide (GM3). Osteoblastic potential was indicated by high levels of alkaline phosphatase and collagen I, expression of osteopontin and osteonectin (SPARC), and production of bone-specific osteocalcin and hydroxyapatite. Cultures of these cells were stimulated to form increased numbers of calcium-mineral-producing nodules by the oxysterol 25-hydroxycholesterol as well as by transforming growth factor-beta 1, both known to be present in atherosclerotic lesions. The stimulation of calcifying vascular cells in the artery wall by these two factors suggests a possible mechanism for the colocalization of calcification with atherosclerosis in vivo. Topics: Alkaline Phosphatase; Animals; Aorta; Arteriosclerosis; Biomarkers; Calcinosis; Cattle; Clone Cells; Cytokines; G(M3) Ganglioside; Histocytochemistry; Humans; Hydroxycholesterols; Osteoblasts; Osteocalcin; Transforming Growth Factor beta	1994

Article

Year

TGF-beta 1 and 25-hydroxycholesterol stimulate osteoblast-like vascular cells to calcify.

The Journal of clinical investigation, 1994, Volume: 93, Issue:5

Previous studies in our laboratory demonstrated messenger RNA for bone morphogenetic protein-2a in human calcified plaque, suggesting that arterial calcification is a regulated process, similar to osteogenesis. To further test this hypothesis, we have isolated and cloned a subpopulation of cells from bovine aortic media that show osteoblastic potential. These novel cells are primarily distinguished from smooth muscle cells by expression of a surface marker preliminarily identified as a modified form of the ganglioside sialyl-lactosylceramide (GM3). Osteoblastic potential was indicated by high levels of alkaline phosphatase and collagen I, expression of osteopontin and osteonectin (SPARC), and production of bone-specific osteocalcin and hydroxyapatite. Cultures of these cells were stimulated to form increased numbers of calcium-mineral-producing nodules by the oxysterol 25-hydroxycholesterol as well as by transforming growth factor-beta 1, both known to be present in atherosclerotic lesions. The stimulation of calcifying vascular cells in the artery wall by these two factors suggests a possible mechanism for the colocalization of calcification with atherosclerosis in vivo.

Topics: Alkaline Phosphatase; Animals; Aorta; Arteriosclerosis; Biomarkers; Calcinosis; Cattle; Clone Cells; Cytokines; G(M3) Ganglioside; Histocytochemistry; Humans; Hydroxycholesterols; Osteoblasts; Osteocalcin; Transforming Growth Factor beta

1994