g(m1)-ganglioside and Intestinal-Neoplasms

g(m1)-ganglioside has been researched along with Intestinal-Neoplasms* in 1 studies

Other Studies

1 other study(ies) available for g(m1)-ganglioside and Intestinal-Neoplasms

Article	Year
Biosynthesis of Lewis antigenic glycolipid by cell-free extracts of human intestinal tumor cells cultured in serum-free medium. Biochemical and biophysical research communications, 1985, Sep-16, Volume: 131, Issue:2 Extracts of the human intestinal tumor cell line SW1116 were able to stimulate the incorporation of (14C) fucose from GDP-(14C) fucose into organically soluble glycolipid. The reaction required a purified glycolipid preparation from human meconium as lipid acceptor. The active glycolipid co-migrated with standard globoside on high performance thin-layer chromatography (HPTLC) and had molecular species (M + H) under fast-atom bombardment mass spectrometry of 1199, 1245 and 1269. Globoside itself was inactive and asialo GM1b had low activity. The radioactive products co-purified with Lewis a and Lewis b and co-migrated principally (60-90%) with Lewis b monoclonal antibody binding cellular glycolipids on HPTLC. Analysis of fucosidase digests suggested the presence of two different fucosyl-hexose linkages one of which was susceptible to cleavage. We conclude that the data are consistent with fucosylation of lactotetraosyl ceramide to Lewis a and Lewis b antigenic glycolipids. Topics: Antibodies, Monoclonal; Cell Line; Cell-Free System; Chromatography, High Pressure Liquid; Fucose; G(M1) Ganglioside; Globosides; Glycolipids; Glycosphingolipids; Humans; Intestinal Neoplasms; Lewis Blood Group Antigens; Mass Spectrometry; Meconium; Molecular Weight; Taurocholic Acid	1985

Article

Year

Biosynthesis of Lewis antigenic glycolipid by cell-free extracts of human intestinal tumor cells cultured in serum-free medium.

Biochemical and biophysical research communications, 1985, Sep-16, Volume: 131, Issue:2

Extracts of the human intestinal tumor cell line SW1116 were able to stimulate the incorporation of (14C) fucose from GDP-(14C) fucose into organically soluble glycolipid. The reaction required a purified glycolipid preparation from human meconium as lipid acceptor. The active glycolipid co-migrated with standard globoside on high performance thin-layer chromatography (HPTLC) and had molecular species (M + H) under fast-atom bombardment mass spectrometry of 1199, 1245 and 1269. Globoside itself was inactive and asialo GM1b had low activity. The radioactive products co-purified with Lewis a and Lewis b and co-migrated principally (60-90%) with Lewis b monoclonal antibody binding cellular glycolipids on HPTLC. Analysis of fucosidase digests suggested the presence of two different fucosyl-hexose linkages one of which was susceptible to cleavage. We conclude that the data are consistent with fucosylation of lactotetraosyl ceramide to Lewis a and Lewis b antigenic glycolipids.

Topics: Antibodies, Monoclonal; Cell Line; Cell-Free System; Chromatography, High Pressure Liquid; Fucose; G(M1) Ganglioside; Globosides; Glycolipids; Glycosphingolipids; Humans; Intestinal Neoplasms; Lewis Blood Group Antigens; Mass Spectrometry; Meconium; Molecular Weight; Taurocholic Acid

1985