fusarin-c and Cell-Transformation--Neoplastic

Fusarin C (FC) is a mutagenic and carcinogenic mycotoxin which was isolated from Fusarium moniliforme culture extracts. The Fusarium moniliforme is one of most prevalent fungi found on corn in Linxian, a high risk area for esophageal cancer. This paper reports, for the first time, the malignant transformation of rat esophageal epithelial cells induced by FC. The transformed cells showed several characteristics of transformation. Colonies were formed after seeding these transformed cells either into selective medium free of epidermal growth factor (EGF) and serum, or on semi-solid agar; there was an increase in chromosome number; the expression of c-myc and v-erb-B oncogenes was enhanced in the cells; and squamous cell carcinomas arose after inoculating the cells into nude mice. The results demonstrated transforming effect of FC on rat esophageal epithelial cells, and indicate that the abnormal expression of some oncogenes could serve as a new property of transformed cells.

Topics: Animals; Cell Line; Cell Transformation, Neoplastic; Epithelial Cells; Esophageal Neoplasms; Esophagus; Gene Expression Regulation, Neoplastic; Genes, myc; Mice; Mice, Nude; Mutagens; Oncogene Proteins v-erbB; Oncogenes; Polyenes; Rats; Rats, Inbred Strains; Retroviridae Proteins, Oncogenic; Tetradecanoylphorbol Acetate