furostanol-i and Stomach-Neoplasms
furostanol-i has been researched along with Stomach-Neoplasms* in 1 studies
Other Studies
1 other study(ies) available for furostanol-i and Stomach-Neoplasms
Article | Year |
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Protodioscin isolated from fenugreek (Trigonella foenumgraecum L.) induces cell death and morphological change indicative of apoptosis in leukemic cell line H-60, but not in gastric cancer cell line KATO III.
Protodioscin (PD) was purified from fenugreek (Trigonella foenumgraecum L.) and identified by Mass, and 1H- and 13C-NMR. The effects of PD on cell viability in human leukemia HL-60 and human stomach cancer KATO III cells were investigated. PD displayed strong growth inhibitory effect against HL-60 cells, but weak growth inhibitory effect on KATO III cells. Morphological change showing apoptotic bodies was observed in the HL-60 cells treated with PD, but not in KATO III cells treated with PD. Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 75.2, 96.3, and 100% after a 3-day treatment with 2.5, 5, and 10 microM PD, respectively. The fragmentation by PD of DNA to oligonucleosomal-sized fragments, that is a characteristic of apoptosis, was observed to be both concentration- and time-dependent in the HL-60 cells. These findings suggest that growth inhibition by PD of HL-60 cells results from the induction of apoptosis by this compound in HL-60 cells. Topics: Apoptosis; Carbohydrate Conformation; Carbohydrate Sequence; Cell Death; Cell Survival; Diosgenin; DNA, Neoplasm; Drugs, Chinese Herbal; HL-60 Cells; Humans; Kinetics; Lymphocytes; Molecular Sequence Data; Oligosaccharides; Saponins; Stomach Neoplasms; Trigonella; Tumor Cells, Cultured | 2003 |