fura-pe3 has been researched along with Hemolysis* in 1 studies
1 other study(ies) available for fura-pe3 and Hemolysis
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[Effect of discodermin A, an antimicrobial peptide, on the cytoplasm membrane].
The effects of discodermin A, an antimicrobial peptide extracted from sea sponge Discodermia kiiensis, on cell membranes were investigated using vascular smooth muscle cells and erythrocytes. At lower concentrations (0.1-3 microM), discodermin A increased muscle tension with an increase in intracellular free Ca2+ concentration ([Ca2+]i) indicated by the fluorescence of Ca2+ indicator, fura-PE3, in rat aortic smooth muscle. On the other hand, the higher concentration of discodermin A (10-30 microM) accelerated the leakage of loaded fura-PE3 from cells. In rabbit mesenteric artery treaded with discodermin A, addition of micromolar concentration of Ca2+ evoked contraction in the presence of ATP, suggesting that permeability of the membrane to Ca2+ and ATP is increased by discodermin A. Confocal fluorescence microscopy showed that discodermin A permeabilized the plasma membrane of A10 cells to fluorescent agents EthD-1 and the intracellular esterase coupled with another fluorescent agent calcein. Discodermin A also showed a hemolytic effect on rabbit erythrocytes, suggesting that discodermin A permitted transmembrane passage of hemoglobin. These results suggest that discodermin A form pores of different sizes on the cytoplasm membrane in concentration- and time-dependent manners. Discodermin A may be a saponin-like bioactive peptide. Topics: Amphibian Proteins; Animals; Antimicrobial Cationic Peptides; Calcium; Cell Membrane Permeability; Fura-2; Hemolysis; In Vitro Techniques; Male; Muscle Contraction; Muscle, Smooth, Vascular; Peptides; Porifera; Rabbits; Rats; Rats, Wistar | 1997 |