fumonisin-b1 and Hepatitis-B

Here, we for the first time used a thiolated amino-ligand modified multi-branched gold nanoflower as skeleton to encapsulate iron porphyrins (AuNF@FeTPPCl) as alternatives to horseradish peroxidase (HRP). After the FeTPPCl encapsulation, the HRP mimicking activity of FeTPPCl was effectively blocked by the steric hindrance of the hydrophobic layer on the AuNF surface. Upon the addition of ethanol, the loaded FeTPPCl was released into the solution in its free format and exposed the catalytic sites, resulting in the recovery of catalytic activity. The proposed encapsulation method effectively avoided the loss of catalytic activity that originated from the blocking of the catalytic active sites during immobilization. Additionally, the resultant AuNF@FeTPPCl nanocomposite exhibited a high loading level with 2.4 × 10

Topics: Antibodies, Immobilized; Biomimetic Materials; Biosensing Techniques; Colorimetry; Fumonisins; Gold; Hepatitis B; Hepatitis B Surface Antigens; Hepatitis B virus; Horseradish Peroxidase; Humans; Immunoassay; Metal Nanoparticles; Metalloporphyrins