fumonisin-b1 and Diarrhea

To study the individual and combined effects of fumonisin B1 (FB1) toxicity and Salmonella serotype Gallinarum infection, Japanese quail (Coturnix coturnix japonica) were fed Fusarium moniliforme culture material (2.5%), 150 mg FB1/kg ration, and were subsequently challenged orally with Salmonella Gallinarum organisms (2 x 10(4) colony-forming units) at 21 days of age. The chicks were fed culture material containing FB1 from day 5 till the end of the experiment. After being infected with Salmonella Gallinarum, observations were made 1, 2, 3, 5, 7, 10, 14, and 21 days postinfection. The clinical signs of diarrhea with bloody discharges were more pronounced in the Salmonella-infected birds on the FB1 diet. Mortality caused by Salmonella Gallinarum increased by 12% in the presence of FB1. Mean body weights in both the Salmonella-infected and FB1-fed groups were significantly lower than those of the controls at almost all intervals. Mean values of hemoglobin, packed cell volume, and total erythrocyte count were slightly higher in birds fed FB1 but were lower in the Salmonella Gallinarum groups fed FB1 and plain chick mash. Anemia was evident, between 5 and 10 days postinfection, in quail chicks infected with Salmonella Gallinarum alone. Total leukocyte counts were higher in Salmonella-infected and FB1-fed groups because of an increase in the number of heterophils and lymphocytes. However, the increase in lymphocyte response to infection was lower by 4.27%-30.09% between 3 and 21 days postinfection in the FB1-fed chicks compared with chicks infected with Salmonella Gallinarum. Alanine transaminase and total serum protein were slightly higher in both the infected and FB1-fed groups. This study revealed that the continuous presence of fumonisins in the diets of quail chicks might increase the susceptibility to or the severity of Salmonella Gallinarum infection.

Topics: Animals; Blood Chemical Analysis; Coturnix; Culture Media; Diarrhea; Fumonisins; Fusarium; Hematologic Tests; Immunity, Cellular; India; Poultry Diseases; Salmonella Infections, Animal; Time Factors

Unseasonal rains beginning in 1995 damaged the maize and sorghum crops harvested in a few villages of the Deccan plateau in India. Human consumption of those grains resulted in a foodborne disease outbreak characterized by abdominal pain, borborygmi and diarrhea.. A rapid epidemiological survey was conducted in the affected villages and a detailed house to house survey in selected villages.. People in 27 out of 50 villages surveyed were affected and disease was seen only in households and subjects consuming the rain damaged moldy sorghum or maize. The disease was self limiting. Diarrhea was reproduced in day old cockerels fed contaminated grains from affected households. All 20 sorghum and 12 maize samples collected from affected households had Fusarium sp. as the dominant mycoflora and contained fumonisin B1 in the range of 0.14-7.8 mg/kg and 0.25-64.7 mg/kg, respectively. In contrast, samples collected from unaffected households had fumonisin B1 in low levels ranging from 0.07-0.36 mg/kg and 0.05-0.24 mg/kg, respectively.. The higher water activity in the grains left in the field following harvest led to the production of high levels of fumonisin B1 and consumption of such grains by humans resulted in the disease.

Topics: Abdominal Pain; Adolescent; Adult; Animals; Carboxylic Acids; Carcinogens, Environmental; Chickens; Child; Child, Preschool; Chromatography, High Pressure Liquid; Cohort Studies; Data Collection; Diarrhea; Disease Outbreaks; Edible Grain; Female; Food Microbiology; Foodborne Diseases; Fumonisins; Humans; India; Infant; Male; Mycotoxins; Zea mays