fumarates and Carcinoma--Ehrlich-Tumor

Fumaric acid, isolated as the active component of Capsella bursa-pastoris herb for inhibiting the solid growth of Ehrlich tumor in mice, was found to reduce markedly the growth and viability of Ehrlich, MH134, and L1210 mouse tumor cells in culture at concentration of 0.3 approximately 1.2 mg/ml. In contrast, fumaric acid at these concentrations in the culture medium had no deleterious effect on the monolayer development of mouse and chick embryo cells but exhibited activity to enhance the recovery of the cells from the toxic effects of mitomycin C, aflatoxin B1, N-methyl-N'-nitro-N-nitrosoguanidine, and potassium 1-methyl-7-[2-(5-nitro-2-furyl) vinyl]-4-oxo-1,4-dihydro-1,8-naphthyridine-3-carboxylate.

Topics: Animals; Antineoplastic Agents; Antioxidants; Carcinoma, Ehrlich Tumor; Cell Division; Cell Survival; Cells, Cultured; Chick Embryo; Dicarboxylic Acids; DNA, Neoplasm; Embryo, Mammalian; Fumarates; Leukemia L1210; Liver; Mice; Mice, Inbred Strains; Neoplasms, Experimental

Topics: Animals; Carcinoma, Ehrlich Tumor; Cell Nucleus; DNA; Fumarates; Male; Mice; Mice, Inbred ICR; Mitomycins; Organ Size; Orotic Acid; RNA; Thymidine

The treatment of ICR mice with i.p. injections (0.14 g/kg/day) of the extract of Capsella bursa-pastoris herb (Cruciferae) caused 50 to 80% inhibition of the solid growth of Ehrlich tumor cells that had been inoculated into the s.c. tissue of the animals. The tumor lumps in the treated mice showed multifocal necroses and the infiltartion of host fibrous tissue cells. Experiments were also performed to isolate and identify the active component for the antitumor action, and an acidic substance was isolated in crystalline form from the herb extract. This acidic substance was identified as fumaric acid and was effective in inhibiting the growth of Ehrlich solid tumor at a dose of 10 mg/kg/day. The 50% lethal dose (i.p.) of this acid was 266 mg/kg.

Topics: Animals; Carcinoma, Ehrlich Tumor; Fumarates; Magnoliopsida; Mice; Plants, Medicinal

Topics: Adenine Nucleotides; Adenosine Monophosphate; Amides; Animals; Binding Sites; Binding, Competitive; Carcinoma, Ehrlich Tumor; Chromatography, Ion Exchange; Feedback; Fumarates; Imidazoles; Kinetics; Lyases; Mathematics; Ribonucleotides; Succinates

Topics: Animals; Carcinoma, Ehrlich Tumor; Citrates; Citric Acid Cycle; Culture Techniques; Depression, Chemical; Drug Synergism; Fumarates; Glucose; Ketoglutaric Acids; Lactates; Malates; Oxaloacetates; Oxygen Consumption; Pyruvates; Stimulation, Chemical; Succinates; Tetracycline

Topics: Alkylating Agents; Animals; Antineoplastic Agents; Azaguanine; Carcinoma, Ehrlich Tumor; Fumarates; Glucuronates; Iron; Mercaptopurine; Mice; Thiotepa

Topics: Animals; Antimycin A; Butyrates; Carcinoma, Ehrlich Tumor; Citrates; Ethanol; Female; Fumarates; Glucose; Glutamates; Glycolysis; Hexokinase; Hexosephosphates; In Vitro Techniques; Ketoglutaric Acids; Lymphoma; Malates; Mice; Neoplasms, Experimental; Nitrogen; Oxygen Consumption; Rotenone

Topics: Animals; Antimetabolites; Antineoplastic Agents; Ascites; Carboxylic Acids; Carcinoma, Ehrlich Tumor; Citric Acid Cycle; Cyclopentanes; Cytochromes; Dinitrophenols; Electron Transport Complex II; Electron Transport Complex IV; Enzyme Inhibitors; Fumarates; Malate Dehydrogenase; Manometry; Metabolism; Mice; Mitochondria; NAD; Neoplasms, Experimental; Oxidoreductases; Pharmacology; Pyruvates; Research; Succinate Dehydrogenase

Topics: Animals; Ascites; Carbohydrate Metabolism; Carcinoma, Ehrlich Tumor; Citric Acid Cycle; Dicumarol; Fluorescence; Fluorometry; Fumarates; Glucose; Glycolysis; Histocytochemistry; Malonates; Mitochondria; Nucleotides; Oxidation-Reduction; Pharmacology; Pyridines; Research; Succinates; Succinic Acid; Tissue Culture Techniques