fraxetin and Stroke

Fraxetin has antioxidant, anti-inflammation and neuroprotective functions, however, its role in ischemic stroke is still vague. Herein, this study delves into the underlying mechanism.. Ischemia and reperfusion operation were performed to establish the cerebral stroke rat models. The brain functions were evaluated with neurological score. The brain infarcted volume in fraxetin group was measured by 2,3,5-triphenyltetrazolium chloride staining. The blood-brain barrier permeability, CD34 enrichment, and the brain water content were measured by Evans blue staining, immunofluorescence staining, and wet-dry method, respectively. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blot (WB) were applied to examine the levels of angiogenesis- and PI3K/Akt pathway-related factors. MTT and tube formation assays were used to measure the viability and tube formation of HUVECs.. Fraxetin decreased the brain injury-related neurological score, brain infarction, and cerebral edema and maintained blood-brain barrier permeability, whereas it promoted the angiogenesis in ischemia-damaged brain. Fraxetin has protective effects on the brain ischemia-reperfusion injury and promotes angiogenesis for cerebral repair

Topics: Animals; Brain; Brain Ischemia; Coumarins; Infarction, Middle Cerebral Artery; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt; Rats; Reperfusion Injury; Signal Transduction; Stroke