fosfomycin and Poultry-Diseases

The objective of this study was to determine, in broilers, which modality of disodium fosfomycin (DF) administration and at what dose the best pharmacokinetic (PK) profile could be obtained, taking as reference a 110 field bacterial strains of Escherichia coli minimum inhibitory concentration survey. The DF was administered via drinking water either ad libitum or at a higher concentration having 1 h of water restriction to build up thirst in the birds (loading dose). Dosages tested were 10, 20, 40, and 80 mg/kg per administration, either once or twice daily. Birds included were 24-d-old Cornish broilers randomly assigned to 16 groups of 200 birds per group and 3 replicates per group. The PK of DF was determined after ad libitum administration of either a single- or double-loading dose or after an initial loading dose followed by ad libitum medication. Also, PK after i.v. administration was studied in separate groups. Serial blood samplings were performed in all groups. Serum obtained was analyzed for DF and a possible active metabolite by means of a microbiological agar diffusion assay. The DF showed a short elimination half-life (approximately 2 h after oral loading administration) with a rapid clearance (1.23 to 1.42 mL/kg per h). Apparent volume of distribution-area under the curve values were also low (10 and 80 mg/kg=0.25 L/kg and 0.22 L/kg, respectively). Considering a minimum inhibitory concentration level that inhibited 90% of total strains of 8 µg/mL for E. coli, it is concluded that single-loading administration at 10, 20, 40, and 80 mg/kg complies poorly with sustained serum concentrations over a dosing interval of 24 h. Doses of 10 and 20 mg/kg twice a day also were insufficient to attain therapeutic concentrations. Useful serum concentrations of DF to treat outbreaks of susceptible E. coli require an initial loading dose of 40 mg/kg, followed by an ad libitum medication of 40 mg/kg 8 h later (80 mg/kg per d).

Topics: Animals; Area Under Curve; Chickens; Dose-Response Relationship, Drug; Escherichia coli; Escherichia coli Infections; Fosfomycin; Half-Life; Microbial Sensitivity Tests; Poultry Diseases; Protein Binding; Time Factors

Two trials were made to assess the efficacy of including calcium fosfomycin in the drinking water or in the feed for four days to control the adverse effects of experimentally induced colibacillosis in broiler chickens. Trial 1 had five groups of 15 chicks each: one group of negative controls; an untreated infected control group and three groups treated with 50, 100 or 200 ppm of calcium fosfomycin in drinking water. Trial 2 had the same groups but the antibiotic was incorporated into the feed. The chickens were infected via their air sacs with 1.7 x 10(8) - 3.6 x 10(8) CFU/chick of Escherichia coli O78:K80. The morbidity and mortality, a score for the gross lesions, the relative weight of the liver and spleen, performance and re-isolation of the challenge bacteria were recorded. Calcium fosfomycin in the drinking water controlled the colibacillosis, particularly in the group treated with 200 ppm. However, no effect of the antibiotic was seen when calcium fosfomycin was incorporated into the feed, the mortality, score of lesions and re-isolation of E. coli from the organs in the three treated groups being similar to those for the infected unmedicated group. The amount of antibiotic ingested in trial I was three times more than in trial 2. These results suggest that calcium fosfomycin is best used in the drinking water for the treatment of colibacillosis.

Topics: Administration, Oral; Animal Feed; Animals; Anti-Bacterial Agents; Body Weight; Chickens; Escherichia coli; Escherichia coli Infections; Female; Fosfomycin; Liver; Male; Myocardium; Poultry Diseases; Survival Rate; Water

Fosfomycin is a broad-spectrum antibiotic with the activity against both Gram-positive and Gram-negative pathogens by inhibiting the bacterial cell wall synthesis. Given the potential therapeutic efficacy of fosfomycin against Campylobacter spp., the aim of the present study was to determine the in vitro fosfomycin susceptibility of Campylobacter isolates from avian sources including poultry and wild birds. A total of eight (1.8%) strains of Campylobacter including five C. jejuni strains isolated from ducks and three C. coli strains isolated from chickens and duck showed resistance to fosfomycin, with MICs ranging from 64 to ≥ 256 μg/mL. The extent of fosfomycin resistance was 0%, 0.9% and 3.9% in wild birds, chicken and ducks respectively. The MIC50, MIC90, and MIC100 values were 8, 32, and 32 μg/mL respectively in wild bird, 32, 32, and 64 μg/mL respectively in chicken, and 32, 32, and ≥ 256 μg/mL respectively in ducks. All eight fosfomycin-resistant Campylobacter strains were multidrug resistant; six were also resistant to fluoroquinolones, ampicillin, and tetracycline, and two were also resistant to fluoroquinolones, ampicillin, tetracycline, and macrolides. However, the fosfomycin resistance gene fosXCC was not detected in the eight fosfomycin-resistant strains. Because food animals can harbor fosfomycin-resistant Campylobacter and transmit them to humans, greater efforts are needed to monitor the prevalence of fosfomycin resistance in Campylobacter strains isolated from such animals.

Topics: Animals; Animals, Wild; Anti-Bacterial Agents; Birds; Campylobacter; Campylobacter Infections; Chickens; Drug Resistance, Multiple, Bacterial; Ducks; Electrophoresis, Gel, Pulsed-Field; Fosfomycin; Inhibitory Concentration 50; Microbial Sensitivity Tests; Poultry; Poultry Diseases

We previously described

Topics: Amino Acid Sequence; Animals; Anti-Bacterial Agents; Chickens; Disk Diffusion Antimicrobial Tests; Drug Resistance, Bacterial; Fosfomycin; Glutathione Transferase; Poultry Diseases; Salmonella enterica

The novel 63,558-bp plasmid pSA-01, which harbors nine antibiotic resistance genes, including

Topics: Animals; Anti-Bacterial Agents; Bacterial Proteins; Chickens; China; Drug Resistance, Multiple, Bacterial; Farms; Fosfomycin; High-Throughput Nucleotide Sequencing; Microbial Sensitivity Tests; Plasmids; Poultry Diseases; Staphylococcal Infections; Staphylococcus

Topics: Animals; Anti-Bacterial Agents; Base Sequence; beta-Lactamases; Chickens; China; Conjugation, Genetic; DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Fosfomycin; Genotype; Humans; Methyltransferases; Molecular Sequence Data; Plasmids; Poultry Diseases; Public Health; Sequence Analysis, DNA

One hundred and twenty 1-day-old broiler chickens were divided into four groups: group I unmedicated and orally challenged with 1.5 x 10(8) cfu of Salmonella enteritidis phage type 4; group F infected and treated with 300 ppm of fosfomycin in their drinking water; group CF uninfected and treated, and group C maintained as a control group. Their performance, clinical signs, S. enteritidis PT4 reisolation and biochemistry variables were compared. Group F showed fewer symptoms and gross lesions than those from group 1. Fosfomycin treatment at 300 ppm improved body weight at 7 days of age by 42.3%. S. enteritidis PT4 reisolation in group I was higher than in the treated group, but total decontamination of challenged birds was not achieved. There was an increase in the levels of total protein and globulins in group I but not in the treated group. Fosfomycin caused no adverse effects on chickens from group CF, assessed by performance and biochemical variables. The results indicate that fosfomycin could be used in the treatment of S. enteritidis PT4 experimental infection.

Topics: Animals; Anti-Bacterial Agents; Bacteriophage Typing; Body Weight; Cecum; Chickens; Colony Count, Microbial; Fosfomycin; Liver; Poultry Diseases; Salmonella enteritidis; Salmonella Infections, Animal; Salmonella Phages; Spleen; Time Factors

This study on the microbiology of chicken assessed a total of 97 live birds from three selected farms and 87 carcasses/chicken parts from two supermarkets, two open markets and one wholesale outlet (cold store) in the Accra metropolis. Campylobacter spp. were isolated from 14 (14.4%) gut contents of live birds from three farms. None of the frozen birds were positive for Campylobacter spp. These isolates were sensitive to most common antibiotics but not to ampicillin and tetracyclines. Salmonella spp. were isolated from 7 (7.2%) gut contents and 13 (6.8%) carcasses and were resistant to erythromycin. cefotiam, penicillin, ampicillin and cefadroxil. Samonella spp. had varied susceptibilities to nalidixic acid, chloramphenicol and minocyclin. No Shigella spp. was isolated from any of the live birds but 6 (6.9%) of imported chicken samples from the cold store and two markets were positive. Fosfomycin and chloramphenicol were the only antibiotics effective against these isolates. Twelve different Escherichia coli serovars were identified from the total of 21 positive samples. These, in order of magnitude isolated, are E. coli 0158 (14.3%), 0125 (14.3%), 025 (9.5%), 028ac (9.5%), 0159 (9.5%). 015 (9.5%), 0126 (9.5%), 063 (4.8%), 0143 (4.8%), 026 (4.8%), 078 (4.8%), 0164 (4.8%). Cefadroxil, ampicillin, penicillin, cefotiam, tetracycline and erythromycin were ineffective against all strains of E. coli isolated. Minocyclin was effective against all strains with the exception of E. coli 0159, 025, 0164 and 063, which were moderately susceptible. All strains with exception of E. coli 0164 were susceptible to fosfomycin. Nalidixic acid, chloramphenicol, kanamaycin, ceftrioxone and amoxycillin all showed varied effectiveness against the strains isolated. It is concluded that imported and locally produced chicken is a potential source of multiple-antibiotic-resistant enteropathogenic bacteria. Measures to improve the microbial quality of chicken are discussed.

Topics: Animals; Anti-Bacterial Agents; Bacterial Typing Techniques; Campylobacter; Chickens; Colony Count, Microbial; Drug Resistance, Bacterial; Escherichia coli; Food Contamination; Food Microbiology; Fosfomycin; Meat; Microbial Sensitivity Tests; Poultry Diseases; Salmonella; Shigella