formazans and Root-Resorption

Survival of periodontal ligament cells is crucial issue after tooth replantation. To understand this matter, we introduced MTT assay, which can be used as a tool for measuring the viability of periodontal ligament cells from extracted rat molars.. The maxillary molars of 4-week-old Sprague-Dawley white female rats were used. Ten teeth of each immediate, 1 h Viaspan and 1 h dry were processed for MTT evaluation. Another 10 teeth from each group were treated in the same manner as above, but were replanted into their original socket. After 2 weeks, the animals were killed and the prevalence of resorption pits was evaluated.. In vivo MTT assay corresponded with the histological results of the resorption pits (P

Topics: Animals; Cell Survival; Female; Formazans; Indicators and Reagents; Molar; Periodontal Ligament; Rats; Rats, Sprague-Dawley; Root Resorption; Tooth Replantation