formazans and Neoplasm-Metastasis

formazans has been researched along with Neoplasm-Metastasis* in 2 studies

Other Studies

2 other study(ies) available for formazans and Neoplasm-Metastasis

ArticleYear
Establishment and characteristics of two syngeneic human osteosarcoma cell lines from primary tumor and skip metastases.
    Acta pharmacologica Sinica, 2008, Volume: 29, Issue:3

    To characterize and compare the different biological behaviors of 2 novel human osteosarcoma cell lines, Zos and Zos-M, established respectively from the primary tumor and the skip metastasis of an osteosarcoma patient.. In vitro studies included morphological observations, karyotype analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay, and cell sensitivity to chemotherapeutic drugs. Subcutaneous and intravenous inoculations into nude mice were carried out to study the tumorigenicity and the metastatic potential. RT-PCR was performed to assess the expression of the osteoblastic markers and some metastasis-related genes.. Both cell lines remained stable for more than 100 passages in vitro without interruption. The RT-PCR examination indicated that they retained the molecular characteristics of an osteoblastic lineage. The karyotype analysis displayed aneuploidy and various structural abnormalities. Both cell lines are tumorigenic; Zos-M differs from Zos by the former's ability to develop lung metastasis after intravenous injection. The comparison of the expression patterns of some metastasis-related genes revealed that the decreased expression of cadherin-11 in Zos-M may correlate with a high potential of metastases. Moreover, both cell lines are less sensitive to the current chemotherapy protocols.. The establishment of osteosarcoma cell lines, Zos and Zos-M, and related animal models provide a useful resource for studying the aggressive behavior of osteosarcoma and will be helpful for screening effective treatment strategies.

    Topics: Adolescent; Animals; Antimetabolites, Antineoplastic; Biomarkers, Tumor; Cell Line, Tumor; Cell Lineage; Cell Proliferation; Collagen; Drug Combinations; Extracellular Matrix; Formazans; Humans; Inhibitory Concentration 50; Karyotyping; Laminin; Male; Methotrexate; Mice; Mice, Nude; Neoplasm Metastasis; Osteosarcoma; Proteoglycans; Tetrazolium Salts; Xenograft Model Antitumor Assays

2008
Naturally occurring resistance of bone marrow mononuclear and metastatic cancer cells to anticancer agents.
    Clinical & experimental metastasis, 2006, Volume: 23, Issue:5-6

    Numerous cancer patients fail standard chemotherapy or develop resistance to chemotherapy during the course of treatment. The purpose of this study is to elucidate the overall response of cells obtained from cancer patients and from normal individuals to chemotherapeutic agents. We analysed the chemosensitivity of cancer cells derived from bone marrow and from pleural effusions or ascites fluids from patients with different cancers. Chemosensitivity to doxorubicin, cisplatin and paclitaxel was determined using the MTT assay. We also determined the response of bone marrow mononuclear (BMMN) cells. There was a wide range of responses to chemotherapy drugs in samples from different individuals. This was observed in cells derived from bone marrow and from ascites or pleural fluids. Large variations were also observed among morphologically normal BMMN cells and metastatic cancer cells from chemo-naïve patients. Cancer cells can easily be collected from ascites or pleural fluids and reliably assayed for chemosensitivity. We describe here that inherent chemoresistance may be a reason for the lack of response to chemotherapy in some patients. We discuss the potential of using the determination of natural resistance to dictate the drugs to be employed for treatment.

    Topics: Antineoplastic Agents; Ascitic Fluid; Bone Marrow Cells; Cell Survival; Cisplatin; Dose-Response Relationship, Drug; Doxorubicin; Drug Resistance, Neoplasm; Female; Formazans; Humans; Inhibitory Concentration 50; Male; Neoplasm Metastasis; Neoplasms; Neoplastic Stem Cells; Paclitaxel; Pleural Effusion, Malignant; Tetrazolium Salts

2006
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