formazans and Cholangitis

Study of the functional activity of blood and biliary neutrophils in patients with purulent cholangitis revealed different patterns of blood neutrophil reaction in the NBT test. The share of type 1 cells (with diffuse staining of the cytoplasm) in spontaneous and stimulated NBT test is a constant value in the studied patient population. The count of active blood neutrophils (type 2, with formasane granules) depends on the antigen dose in the test. A more grave form (with strictures of the bile duct) is associated with a decreased count of formasane-positive type 2 cells in comparison with that in patients with choledocholithiasis. An accurate and rapid method for the diagnosis of purulent cholangitis has been developed, based on measuring the functional activity of A bile neutrophils collected during surgery. The C bile neutrophils differ from blood neutrophils of patients with purulent cholangitis by morphology and function. Biliary neutrophils reduce the NBT after the diffuse punctuate type. The detected differences are presumably due to specific features of the environment.

Topics: Bile; Cell Movement; Cholangitis; Diagnosis, Differential; Formazans; Humans; Indicators and Reagents; Leukocyte Count; Neutrophils; Nitroblue Tetrazolium; Reproducibility of Results; Suppuration