formazans and Burns

To propose an experimental burn model in NIH-3T3 cell line.. Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and immunofluorescence.. The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.. The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.

Topics: Animals; Burns; Cell Culture Techniques; Cell Survival; Disease Models, Animal; Fluorescent Antibody Technique; Formazans; Hot Temperature; In Vitro Techniques; Mice; Microscopy, Confocal; NIH 3T3 Cells; Reproducibility of Results; Tetrazolium Salts; Time Factors

The phagocytic function of neutrophils has been studied in 50 Egyptians of both sexes and of various ages, suffering from burns of different depths and extents, and the results compared with data from 50 normal persons of similar sex and age. The nitro blue tetrazolium test (Gifford and Malawista, 1970), and the bacterial killing test (Quie et al., 1967), have been used during the first 24 hours, and then repeated at weekly intervals for 4 weeks. The phagocytic power showed no change during the study except in two patients with extensive burns during fatal septicaemia. The bactericidal capacity, and the formazan cell formation showed similar changes with a steady decrease from the first to the third weeks, followed by a rise during the fourth week. Similar changes were noticed in the serum albumin levels. The decline was more marked in burns of more than 50 per cent of the body surface area and during severe sepsis. Improvement coincided with healing.

Topics: Blood Bactericidal Activity; Burns; Female; Formazans; Humans; Male; Neutrophils; Nitroblue Tetrazolium; Phagocytosis; Serum Albumin; Time Factors