fm19g11 and Amyotrophic-Lateral-Sclerosis

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting motor neurons. In ALS mice, neurodegeneration is associated with the proliferative restorative attempts of ependymal stem progenitor cells (epSPCs) that normally lie in a quiescent in the spinal cord. Thus, modulation of the proliferation of epSPCs may represent a potential strategy to counteract neurodegeneration. Recent studies demonstrated that FM19G11, a hypoxia-inducible factor modulator, induces epSPC self-renewal and proliferation. The aim of the study was to investigate whether FM19G11-loaded gold nanoparticles (NPs) can affect self-renewal and proliferation processes in epSPCs isolated from G93A-SOD1 mice at disease onset. We discovered elevated levels of SOX2, OCT4, AKT1, and AKT3, key genes associated with pluripotency, self-renewal, and proliferation, in G93A-SOD1 epSPCs at the transcriptional and protein levels after treatment with FM19G11-loaded NPs. We also observed an increase in the levels of the mitochondrial uncoupling protein (

Topics: Amyotrophic Lateral Sclerosis; Animals; Benzamides; Biomarkers; Cell Cycle; Cell Proliferation; Cell Self Renewal; Ependyma; Gene Expression Regulation; Gold; Humans; Metal Nanoparticles; Mice, Transgenic; MicroRNAs; Octamer Transcription Factor-3; Pluripotent Stem Cells; Proto-Oncogene Proteins c-akt; PTEN Phosphohydrolase; SOXB1 Transcription Factors; Stem Cells; Superoxide Dismutase-1; Uncoupling Protein 2