fm1-43 and Teratocarcinoma

fm1-43 has been researched along with Teratocarcinoma* in 1 studies

Other Studies

1 other study(ies) available for fm1-43 and Teratocarcinoma

Article	Year
Nitric oxide and cyclic nucleotide signal transduction modulates synaptic vesicle turnover in human model neurons. Journal of neurochemistry, 2009, Volume: 111, Issue:6 The human Ntera2 (NT2) teratocarcinoma cell line can be induced to differentiate into post-mitotic neurons. Here, we report that the human NT2 neurons generated by a spherical aggregate cell culture method express increasing levels of typical pre-synaptic proteins (synapsin and synaptotagmin I) along the neurite depending on the length of in vitro culture. By employing an antibody directed against the luminal domain of synaptotagmin I and the fluorescent dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl)pyridinium dibromide, we show that depolarized NT2 neurons display calcium-dependent exo-endocytotic synaptic vesicle recycling. NT2 neurons express the neuronal isoform of neuronal nitric oxide synthase and soluble guanylyl cyclase (sGC), the major receptor for nitric oxide (NO). We tested whether NO signal transduction modulates synaptic vesicle turnover in human NT2 neurons. NO donors and cylic guanosine-monophosphate analogs enhanced synaptic vesicle recycling while a sGC inhibitor blocked the effect of NO donors. Two NO donors, sodium nitroprusside, and and N-Ethyl-2-(1-ethyl-2-hydroxy-2-nitrosohydrazino) ethanamine evoked vesicle exocytosis which was partially blocked by the sGC inhibitor. The activator of adenylyl cyclase, forskolin, and a cAMP analog induced synaptic vesicle recycling and exocytosis via a parallel acting protein kinase A pathway. Our data from NT2 neurons suggest that NO/cyclic nucleotide signaling pathways may facilitate neurotransmitter release in human brain cells. Topics: 1-Methyl-3-isobutylxanthine; 8-Bromo Cyclic Adenosine Monophosphate; Cell Differentiation; Cell Line, Tumor; Colforsin; Cyclic GMP; Enzyme Inhibitors; Exocytosis; Humans; Isoquinolines; Microtubule-Associated Proteins; Neurons; Nitric Oxide; Nitric Oxide Synthase Type I; Nitroso Compounds; Nucleotides, Cyclic; Phosphodiesterase Inhibitors; Pyridinium Compounds; Quaternary Ammonium Compounds; Signal Transduction; Sulfonamides; Synapsins; Synaptic Vesicles; Synaptotagmin I; Teratocarcinoma; Time Factors	2009

Article

Year

Nitric oxide and cyclic nucleotide signal transduction modulates synaptic vesicle turnover in human model neurons.

Journal of neurochemistry, 2009, Volume: 111, Issue:6

The human Ntera2 (NT2) teratocarcinoma cell line can be induced to differentiate into post-mitotic neurons. Here, we report that the human NT2 neurons generated by a spherical aggregate cell culture method express increasing levels of typical pre-synaptic proteins (synapsin and synaptotagmin I) along the neurite depending on the length of in vitro culture. By employing an antibody directed against the luminal domain of synaptotagmin I and the fluorescent dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl)pyridinium dibromide, we show that depolarized NT2 neurons display calcium-dependent exo-endocytotic synaptic vesicle recycling. NT2 neurons express the neuronal isoform of neuronal nitric oxide synthase and soluble guanylyl cyclase (sGC), the major receptor for nitric oxide (NO). We tested whether NO signal transduction modulates synaptic vesicle turnover in human NT2 neurons. NO donors and cylic guanosine-monophosphate analogs enhanced synaptic vesicle recycling while a sGC inhibitor blocked the effect of NO donors. Two NO donors, sodium nitroprusside, and and N-Ethyl-2-(1-ethyl-2-hydroxy-2-nitrosohydrazino) ethanamine evoked vesicle exocytosis which was partially blocked by the sGC inhibitor. The activator of adenylyl cyclase, forskolin, and a cAMP analog induced synaptic vesicle recycling and exocytosis via a parallel acting protein kinase A pathway. Our data from NT2 neurons suggest that NO/cyclic nucleotide signaling pathways may facilitate neurotransmitter release in human brain cells.

Topics: 1-Methyl-3-isobutylxanthine; 8-Bromo Cyclic Adenosine Monophosphate; Cell Differentiation; Cell Line, Tumor; Colforsin; Cyclic GMP; Enzyme Inhibitors; Exocytosis; Humans; Isoquinolines; Microtubule-Associated Proteins; Neurons; Nitric Oxide; Nitric Oxide Synthase Type I; Nitroso Compounds; Nucleotides, Cyclic; Phosphodiesterase Inhibitors; Pyridinium Compounds; Quaternary Ammonium Compounds; Signal Transduction; Sulfonamides; Synapsins; Synaptic Vesicles; Synaptotagmin I; Teratocarcinoma; Time Factors

2009