fluticasone and Staphylococcal-Infections

Here we investigated the relationship between local bacterial colonization and anti-bacterial immune responses in pre-school asthmatic and control children within the EU-wide study PreDicta. In this cohort of pre-school asthmatic children, nasopharyngeal colonization with Gram-negative bacteria such as Haemophilus influenzae and Moraxella catarrhalis was found to be associated with the highest interferon beta (IFNβ) and IL-33 levels in the nasal pharyngeal fluids (NPF). IL33R-ST2 was found induced in the blood of asthmatic children with additional Gram + bacteria in the nasopharynx (Gr+/-). Furthermore, asthmatic children had more episodes of infection that required antibiotic therapy than the control group. Treatment with antibiotics associated with reduced ST2 in blood cells of both asthmatic and control children and reduced IL-33 levels in the airways of asthmatic children. In the absence of Staphylococcus (S.) aureus in NPF, antibiotic therapy associated with decreased IL-33 levels in the NPF and lower ST2 values in the blood of control children but not of asthmatic children. These data suggest that, in asthmatic children, Gram- bacteria, which persist after antibiotic therapy, contributes to IL-33 locally and associated with Gr + bacteria colonization in the airways, inhibited IFN-β and in the absence of Staphylococcus (S.) aureus, induced ST2 bearing cells in their blood.

Topics: Anti-Bacterial Agents; Asthma; Bronchodilator Agents; Case-Control Studies; Child; Child, Preschool; Female; Fluticasone; Gene Expression Regulation; Haemophilus Infections; Haemophilus influenzae; Humans; Interferon-beta; Interleukin-1 Receptor-Like 1 Protein; Interleukin-33; Male; Moraxella catarrhalis; Moraxellaceae Infections; Nasopharynx; Respiratory Function Tests; Salmeterol Xinafoate; Staphylococcal Infections; Staphylococcus aureus

Clinical improvement in patients with chronic rhinosinusitis (CRS) treated with steroids alone has previously been ascribed to the steroids' anti-inflammatory properties rather than any direct effect on the bacteria. The aim of this study was to determine if commonly used intranasal steroids directly reduce bacterial biofilm production in vitro.. In vitro comparative controlled trial.. Staphylococcus aureus biofilms were grown on minimum biofilm eradication concentration device pegs and treated with the commonly prescribed CRS topical steroids fluticasone, mometasone, or budesonide. These were dissolved in vehicle solvents and added to cerebrospinal fluid (CSF) broth. Concentrations (including therapeutic doses) tested for fluticasone and mometasone ranged from 25 μg/200 μL to 400 μg/200 μL, and from 16 μg/200 μL to 2000 μg/200 μL for budesonide. Control pegs were exposed to equivalent volumes of the appropriate solvent/CSF broth. Confocal scanning laser microscopy and COMSTAT software were used to quantify biofilms at 24 hours after treatment.. Significant differences from control were found for fluticasone at 400 μg/200 μL (difference = -0.3065 μm(3)/μm(2), P = .007), mometasone at 300 μg/200 μL and 400 μg/200 μL (difference = -0.15 μm(3)/μm(2), P = .006, and difference = -0.9193 μm(3)/μm(2), P = .034, respectively), and budesonide at 750 μg/200 μL, 1000 μg/200 μL and 2000 μg/200 μL (difference = -1.0137 μm(3)/μm(2), P = .038, difference = -0.6164, P = .009, and difference = -0.1906 μm(3)/μm(2), P = .029, respectively).. The concentrations of 400 μg/200 μL of fluticasone, 300 μg and 400 μg/200 μL of mometasone, and 750 μg, 1,000 μg, and 2,000 μg/200 μL of budesonide directly reduce biofilm production in vitro, outside of the inflammatory milieu.

Topics: Adrenal Cortex Hormones; Androstadienes; Biofilms; Budesonide; Culture Media, Conditioned; Dose-Response Relationship, Drug; Fluticasone; Humans; In Vitro Techniques; Mometasone Furoate; Pregnadienediols; Reference Values; Sensitivity and Specificity; Staphylococcal Infections; Staphylococcus aureus

The aim of this study is to determine the rate of nasal carriage of Staphylococcus aureus (NCSA) in children with allergic rhinitis (AR) and to determine the effect of intranasal fluticasone propionate spray on the NCSA.. Nasal swabs were taken from the children admitted to general pediatrics and pediatric pulmonology clinics. Patients were divided into two groups according to the presence or absence of AR. Diagnosis of AR was based on the patient's symptoms. Nasal swabs were taken from AR patients before and after the treatment with intranasal fluticasone propionate, and from the control group at the beginning and after 2 months.. Whole NCSA rate was 17.9%; it was 21.4% for AR patients and 15.9% for control group, respectively (p>0.05). Treatment with intranasal fluticasone propionate spray did not influence NCSA in AR patients.. It seemed that NCSA was not increased in children with AR and treatment with intranasal fluticasone propionate spray did not change NCSA in AR patients. It is obvious that better understanding of the factors affecting the acquisition and loss of NCSA might increase our knowledge about the relationship between NCSA, allergic airway diseases and their treatments.

Topics: Administration, Intranasal; Androstadienes; Anti-Allergic Agents; Carrier State; Child; Child, Preschool; Female; Fluticasone; Humans; Male; Nose; Prevalence; Rhinitis, Allergic, Seasonal; Staphylococcal Infections; Staphylococcus aureus