fluorapatite and Anthrax

Since bacterial spores, such as Bacillus anthracis spores, are extremely hazardous to human beings and animals, efforts have focused on the development of bacterial spore detector with rapid response and high selectivity and sensitivity. Therefore, we reported a facile one-step chelating-reagent-assisted hydrothermal synthesis of lanthanide-doped fluorapatite (FA) nanoprobes for detecting the biomarker of bacterial spores. In FA synthesis, ethylenediaminetetraacetic acid (EDTA) can serve not only as a shape controller and a stabilizer but also as a chelating reagent for lanthanide ions. After the fabrication, terbium (or europium) ions were not only embedded into lattice models of FA nanocrystals, but also chelated by EDTA on the surface of the FA nanocrystals, which can be available for detecting the biomarker (i.e., dipicolinic acid) of bacterial spores. The obtained FA: Ln-EDTA nanoprobes can provide reference fluorescence which play crucial roles in the calibration and correction of concentration. The fluorescence intensities of FA: Tb-EDTA or FA: Eu-EDTA from Tb

Topics: Anthrax; Apatites; Bacillus anthracis; Biosensing Techniques; Edetic Acid; Fluorescence; Fluorescent Dyes; Humans; Lanthanoid Series Elements; Nanoparticles; Spectrometry, Fluorescence; Spores, Bacterial