flavin-mononucleotide and Pancreatitis

Increased of proinflammatory cytokines levels, including interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) on severe acute pancreatitis causes vasodilatation, increased permeability of the wall, accumulation of fluid in lung tissue and pleural sinuses. Transudate from acute parapancreatyc clusters of hot liquid and abdomen falls into the chest cavity through microscopic defects in the diaphragm due to the formation of pathological pleural-peritoneal connections or the relevant pressure gradient between the abdominal and pleural cavities. Remediation and removal of acute parapancreatyc clusters combined with the use of a multicomponent drug infusion therapy Cytoflavin provide a reduction in the frequency of pulmonary complications of acute pancreatitis from 48.3 to 31.0%. Use of the drug Cytoflavin reduces the severity of endogenous intoxication and mortality from acute lung injury from 12.9 to 6.1%.

Topics: Acute Disease; Acute Lung Injury; Adult; Aged; Antioxidants; Capillary Permeability; Diaphragm; Drug Combinations; Exudates and Transudates; Female; Flavin Mononucleotide; Fluid Therapy; Humans; Inosine Diphosphate; Interleukin-8; Lipid Peroxidation; Lung; Male; Middle Aged; Niacinamide; Pancreas; Pancreatitis; Peritoneum; Pleura; Protective Agents; Succinates; Tumor Necrosis Factor-alpha; Vasodilation

The aims of our experiments on animals were (i) to evaluate by direct oximery the efficiency of various antioxidant drugs in a complex treatment of acute pancreatitis and (ii) to determine the diagnostic value of the direct oximetry method for estimation of the efficiency of medical treatment. The article presents data obtained in a group 75 outbred Guinea with a model acute pancreatitis, which were treated with mexibel (group 1), emoxipin (group 2), end cytoflavin (group 3), with subsequent investigation of the pancreatic tissues by the direct oximetry method. The obtained results confirmed that the intraperitoneal injection of cytoflavin to animals stimulates tissue respiration, improves metabolism, promotes pancreas recovery, and also improves the prognosis and reduces the lethal outcome. The efficiency of cytoflavin within the complex therapy exceeds the effect of other antioxidants (mexibel and emoxipin) under otherwise equal conditions.

Topics: Acute Disease; Animals; Antioxidants; Disease Models, Animal; Drug Combinations; Flavin Mononucleotide; Guinea Pigs; Injections, Intraperitoneal; Inosine Diphosphate; Niacinamide; Oxygen; Oxygen Consumption; Pancreas; Pancreatitis; Partial Pressure; Picolines; Succinates; Treatment Outcome

Major advances in the development and application of the bioluminescent analysis to detect certain biologically active substances are discussed. The main merit of the method lies in its high sensitivity and specificity along with its simplicity and rapid performance. The available methodologies allow for detection of substances of varying nature: Ca2+, ATP, FMN, NAD(P), long-chain aldehydes, ATP- and NAD(P)-dependent enzymes and their substrates, many xenobiotics and antibiotics, and mutagens. The bioluminescence methodologies may be widely applied in clinical laboratory diagnosis.

Topics: Adenosine Triphosphate; Aldehydes; Anti-Bacterial Agents; Firefly Luciferin; Flavin Mononucleotide; Humans; Luciferases; Luminescent Measurements; Models, Biological; Mutagens; NADP; Pancreatitis; Protease Inhibitors; Xenobiotics