flavin-mononucleotide and Neoplasms

Topics: Adenosine Triphosphate; Animals; Azo Compounds; Carcinogens; Carcinoma, Hepatocellular; Flavin Mononucleotide; Flavin-Adenine Dinucleotide; Humans; Liver Neoplasms; Male; Neoplasm Transplantation; Neoplasms; Neoplasms, Experimental; Nucleotidyltransferases; Rats; Riboflavin; Riboflavin Deficiency

Topics: Adult; Animals; Child; Congenital Abnormalities; Female; Flavin Mononucleotide; Flavin-Adenine Dinucleotide; Homeostasis; Humans; Intestinal Absorption; Liver; Neoplasms; Neoplasms, Experimental; Pregnancy; Pregnancy Complications; Rats; Riboflavin; Riboflavin Deficiency; Thyroid Hormones

Riboflavin is an essential vitamin for cellular metabolism and is highly upregulated in metabolically active cells. Consequently, targeting the riboflavin carrier protein (RCP) may be a promising strategy for labeling cancer and activated endothelial cells. Therefore, Ultrasmall SuperParamagnetic Iron Oxide nanoparticles (USPIO) were adsorptively coated with the endogenous RCP ligand flavin mononucleotide (FMN), which renders them target-specific and fluorescent. The core diameter, surface morphology and surface coverage of the resulting FMN-coated USPIO (FLUSPIO) were evaluated using a variety of physico-chemical characterization techniques (TEM, DLS, MRI and fluorescence spectroscopy). The biocompatibility of FLUSPIO was confirmed using three different cell viability assays (Trypan blue staining, 7-AAD staining and TUNEL). In vitro evaluation of FLUSPIO using MRI and fluorescence microscopy demonstrated high labeling efficiency of cancer cells (PC-3, DU-145, LnCap) and activated endothelial cells (HUVEC). Competition experiments (using MRI and ICP-MS) with a 10- and 100-fold excess of free FMN confirmed RCP-specific uptake of the FLUSPIO by PC-3 cells and HUVEC. Hence, RCP-targeting via FMN may be an elegant way to render nanoparticles fluorescent and to increase the labeling efficacy of cancer and activated endothelial cells. This was shown for FLUSPIO, which due to their high T(2)-relaxivity, are favorably suited for MR cell tracking experiments and cancer detection in vivo.

Topics: Cell Line, Tumor; Endothelium, Vascular; Ferric Compounds; Flavin Mononucleotide; Humans; Magnetic Resonance Imaging; Magnetics; Metal Nanoparticles; Microscopy, Electron, Transmission; Neoplasms; Powder Diffraction

Topics: Adaptation, Physiological; Aged; Anesthetics, Combined; Antioxidants; Balanced Anesthesia; Drug Combinations; Female; Flavin Mononucleotide; Humans; Inosine Diphosphate; Lipid Peroxidation; Male; Monitoring, Physiologic; Neoplasms; Neoplastic Processes; Niacinamide; Oxidative Stress; Perioperative Period; Protective Agents; Stress, Physiological; Succinates; Surgical Procedures, Operative; Treatment Outcome

Topics: Adult; Aged; Anesthesia, General; Dose-Response Relationship, Drug; Drug Combinations; Female; Flavin Mononucleotide; Follow-Up Studies; Gynecologic Surgical Procedures; Humans; Hypoxia; Infusions, Intravenous; Inosine Diphosphate; Malondialdehyde; Middle Aged; Neoplasms; Niacinamide; Postoperative Period; Succinates; Treatment Outcome

Topics: Animals; Ascorbic Acid; Cysteamine; Dose-Response Relationship, Radiation; Electron Spin Resonance Spectroscopy; Ferricyanides; Flavin Mononucleotide; Free Radicals; Male; Neoplasms; Organ Specificity; Radiation Effects; Rats; Temperature; Testis; Thymus Gland; Uranium