flavin-adenine-dinucleotide and Uremia

Inhibition of D-amino acid oxidase (DAO) activity by various uremic retention products and guanidino compounds was investigated. Creatinine (CTN) was found to inhibit DAO at a similar concentration in the sera of uremic patients. The inhibition was competitive and the K(i) value was 2.7 mM. Moreover, CTN was shown to interact with flavin adenine dinucleotide (FAD), a coenzyme of DAO. The UV spectral change of FAD bound to DAO was observed in the visible region by addition of CTN. These findings suggest that the increase in serum and tissue CTN concentrations might be responsible, in part, for the increase in D-amino acids in the sera of uremic patients.

Topics: Alanine; Alanine Transaminase; Creatinine; D-Amino-Acid Oxidase; Enzyme Activation; Flavin-Adenine Dinucleotide; Humans; In Vitro Techniques; Serine; Uremia

Topics: Ascorbic Acid; Blood; Carbon Radioisotopes; Enzyme Activation; Erythrocytes; Flavin-Adenine Dinucleotide; Flavins; Glucosephosphate Dehydrogenase Deficiency; Glutathione Reductase; Hemolysis; Humans; Liver Cirrhosis; Methylene Blue; Riboflavin; Umbilical Cord; Uremia

Topics: Adult; Erythrocytes; Flavin-Adenine Dinucleotide; Glucosephosphate Dehydrogenase Deficiency; Glutathione Reductase; Humans; Liver Cirrhosis; Protein Binding; Uremia