flavin-adenine-dinucleotide and Gram-Negative-Bacterial-Infections

The accumulation of chlorophenols (CPs) in the environment, due to their wide use as agrochemicals, has become a serious environmental problem. These organic halides can be degraded by aerobic microorganisms, where the initial steps of various biodegradation pathways include an oxidative dechlorinating process in which chloride is replaced by a hydroxyl substituent. Harnessing these dechlorinating processes could provide an opportunity for environmental remediation, but detailed catalytic mechanisms for these enzymes are not yet known. To close this gap, we now report transient kinetics and product analysis of the dechlorinating flavin-dependent monooxygenase, HadA, from the aerobic organism

Topics: Chlorophenols; Flavin-Adenine Dinucleotide; Gram-Negative Bacterial Infections; Halogenation; Humans; Kinetics; Mixed Function Oxygenases; Ralstonia pickettii; Substrate Specificity