fibrin and Urinary-Incontinence--Stress

Stress urinary incontinence (SUI) is a life changing condition, affecting 20 million women worldwide. In this study, we developed a bioactive, injectable bulking agent that consists of Permacol™ (Medtronic, Switzerland) and recombinant insulin like growth factor-1 conjugated fibrin micro-beads (fib_rIGF-1) for its bulk stability and capacity to induce muscle regeneration. Therefore, Permacol™ formulations were injected in the submucosal space of rabbit bladders. The ability of a bulking material to form a stable and muscle-inducing bulk represents for us a promising therapeutic approach to achieve a long-lasting treatment for SUI. The fib_rIGF-1 showed no adverse effect on human smooth muscle cell metabolic activity and viability in vitro based on AlamarBlue assays and Live/Dead staining. Three months after injection of fib_rIGF-1 together with Permacol™ into the rabbit bladder wall, we observed a smooth muscle tissue like formation within the injected materials. Positive staining for alpha smooth muscle actin, calponin, and caldesmon demonstrated a contractile phenotype of the newly formed smooth muscle tissue. Moreover, the fib_rIGF-1 treated group also improved the neovascularization at the injection site, confirmed by CD31 positive staining compared to bulks made of Permacol

Topics: Animals; Biocompatible Materials; Female; Fibrin; Humans; Immunohistochemistry; Mice; Myocytes, Smooth Muscle; Rabbits; Urinary Incontinence, Stress; Urinary Tract

Transabdominal urethrocystopexy by using a two-component fibrin sealant as a substitute for sutures was performed in 99 women suffering from proved urinary stress incontinence. All were assessed preoperatively, both clinically and by urodynamic tests. This report deals with the results after a follow-up period not shorter than 1 year and up to 4 years. We found it essential not to report postoperative results concerning observation periods shorter than 1 year, as most relapses are observed more than 1 year after operation. Success was defined as absence of objective urine loss at coughing or straining with full bladder in upright position. The results imply that this simple technique is worthy of trial in correcting urinary stress incontinence in women.

Topics: Adult; Aged; Female; Fibrin; Follow-Up Studies; Humans; Middle Aged; Tissue Adhesives; Urethra; Urinary Bladder; Urinary Incontinence, Stress

Topics: Adult; Aged; Biocompatible Materials; Female; Fibrin; Humans; Male; Middle Aged; Prostheses and Implants; Urethra; Urinary Bladder; Urinary Incontinence, Stress

Topics: Biocompatible Materials; Fibrin; Follow-Up Studies; Humans; Prostheses and Implants; Urinary Incontinence, Stress

Topics: Fibrin; Follow-Up Studies; Humans; Urinary Incontinence, Stress

A new simple method of treating urinary stress incontinence is to fix a fibrin implant vaginally to the bladder neck and upper urethra. The support provided by the implant is maintained after its resorption by the fibrous tissue which replaces it. The two-year cure rate in 85 patients was 93 per cent.

Topics: Adult; Aged; Female; Fibrin; Follow-Up Studies; Humans; Male; Middle Aged; Prostheses and Implants; Time Factors; Urethra; Urinary Bladder; Urinary Catheterization; Urinary Incontinence, Stress