fibrin and Stomach-Neoplasms

The purpose of this study was to investigate the effects of acute hypervolemic fluid infusion (AHFI) of 6% hydroxyethyl starch (HES) 130/0.4 or 4% succinylated gelatin (GEL) on hemostasis and the possible mechanisms.. Thirty-six gastric cancer patients were randomized to receive AHFI of either HES, GEL or lactated Ringer's (RL) solution at the rate of 30 mL x kg(-1) x h( -1) from 20 minutes before to 40 minutes after induction of general anesthesia.. Group HES and GEL had significantly prolonged PT and aPTT, decreased VIII:C and vWF immediately after AHFI. Statistically prolonged reaction time and coagulation time, and decreased growth angle were seen immediately after HES infusion. Maximum amplitude decreased significantly in group HES and GEL immediately after and 4 hours after AHFI.. Gelatin reduced clot quality associated with derangements of fibrin polymerization and HES 130/0.4 delayed initiation of sufficient thrombin generation to convert fibrinogen to fibrin and impaired platelet function.

Topics: Adult; Anesthesia, General; Blood Coagulation Tests; Blood Loss, Surgical; Blood Platelets; Blood Volume; Female; Fibrin; Fluid Therapy; Gastrectomy; Gelatin; Hemostasis; Humans; Hydroxyethyl Starch Derivatives; Isotonic Solutions; Male; Middle Aged; Plasma Substitutes; Ringer's Lactate; Stomach Neoplasms; Succinates; Thrombelastography; Thrombin

The development of venous thromboembolism (VTE) is associated with high mortality among gastric cancer (GC) patients. Neutrophil extracellular traps (NETs) have been reported to correlate with the prothrombotic state in some diseases, but are rarely reported in GC patients.. To investigate the effect of NETs on the development of cancer-associated thrombosis in GC patients.. The levels of NETs in blood and tissue samples of patients were analyzed by ELISA, flow cytometry, and immunofluorescence staining. NET generation and hypercoagulation of platelets and endothelial cells (ECs). NETs were likely to form in blood and tissue samples of GC patients compared with healthy individuals.. GC-induced NETs strongly increased the risk of VTE development both

Topics: Animals; Constriction, Pathologic; Endothelial Cells; Extracellular Traps; Fibrin; Mice; Neutrophils; Stomach Neoplasms; Thrombophilia; Thrombosis; Venous Thromboembolism

Topics: Apoptosis; Biocompatible Materials; Calcitriol; Cell Line, Tumor; Delayed-Action Preparations; Fibrin; Fibrin Fibrinogen Degradation Products; Humans; Stomach Neoplasms; Vitamins

The prognosis of gastric cancer during pregnancy is unfavorable because of delayed diagnosis and advanced stage. We present a case of gastric carcinoma metastasized to the placenta and uterus during pregnancy. Pathological examination revealed a poorly differentiated adenocarcinoma of the stomach with lymph node metastasis. After counseling, the patient decided to terminate the pregnancy and begin immediate treatment for gastric cancer. Hysterectomy and subtotal hysterectomy were performed because medical termination of the pregnancy was unsuccessful. Pathological examination of the placenta and uterus revealed metastases of gastric adenocarcinoma. All the uterine vessels were packed with tumor cells and the myometrium showed extensive coagulative necrosis. Moreover, microscopic findings of the placenta were consistent with massive perivillous fibrin deposition. Our case clearly suggests that massive perivillous fibrin deposition in the placenta can be associated with malignancy during pregnancy and that uterine metastasis of maternal malignancy may result in myometrial dysfunction unresponsive to uterotonics.

Topics: Adenocarcinoma; Adult; Chorionic Villi; Female; Fibrin; Humans; Necrosis; Neoplasm Proteins; Placenta; Placental Circulation; Pregnancy; Stomach Neoplasms; Trophoblastic Tumor, Placental Site; Up-Regulation; Uterine Neoplasms; Uterus

Immunohistochemistry was applied to AMeX-fixed tissue sections of 12 adenocarcinomas of the stomach (seven intestinal adenocarcinomas and five diffuse carcinomas), 12 adenocarcinomas of the pancreas (nine ductal adenocarcinomas and three signet ring carcinomas), and 12 squamous cell carcinomas of the larynx obtained at surgical resection to examine the possibility of extravascular activation of blood coagulation in cancer tissues by exploring the in loco patterns of distribution of fibrinogen, a final product of blood coagulation, fibrin, and a by-product of coagulation reactions (prothrombin fragment 1+2). Gastric, pancreatic, and laryngeal cancers exhibited fibrinogen antigen in abundance throughout the tumor stroma. Fibrin was detected along the edges of nests of carcinoma cells and at the host-tumor interface. Prothrombin fragment 1+2 was present in the blood vessels in areas of neoangiogenesis at the host-tumor interface (gastric and pancreatic cancer tissues) and on the tumor cell bodies (pancreatic and laryngeal cancer tissues). The presence of prothrombin fragment 1+2 in cancer tissues appears to be a good indicator of coagulation activation and thrombin generation at the tumor burden.

Topics: Adenocarcinoma; Biomarkers; Blood Coagulation; Carcinoma, Squamous Cell; Fibrin; Gastrointestinal Neoplasms; Immunohistochemistry; Laryngeal Neoplasms; Neovascularization, Pathologic; Pancreatic Neoplasms; Peptide Fragments; Prothrombin; Stomach Neoplasms; Stromal Cells

Chinese herbs nephropathy (CHN), a rapidly progressive interstitial fibrosis of the kidney, has been described in approximately 100 young Belgian women who had followed a slimming regimen containing some Chinese herbs. In 4 patients multifocal transitional cell carcinomas (TCC) were observed. Aristolochic acid (AA), suspected as the causal factor of CHN, is a well known carcinogen but its ability to induce fibrosis has never been demonstrated. The objective of this study was to evaluate the latter using doses of AA, durations of intoxication and delays of sacrifice known to yield tumours in rats. We also tested the hypothesis that a possible fibrogenic role of AA was enhanced by the other components of the slimming regimen. Male and female rats were treated orally with 10 mg isolated AA/kg per day for 5 days/week, or with approximately 0.15 mg AA/ kg per day 5 days/week contained in the herbal powder together with the other components prescribed in the slimming pills for 3 months. The animals were killed respectively 3 and 11 months later. At sacrifice, animals in both groups had developed the expected tumours but not fibrosis of the renal interstitium. Whether the fibrotic response observed in man is due to species and/or strain related differences in the response to AA or to other factors, remains to be determined. Interestingly, despite the addition of fenfluramine and diethylpropion, two drugs incriminated in the development of valvular heart disease, no cardiac abnormalities were observed.

Topics: Animals; Anti-Obesity Agents; Aristolochic Acids; Carcinogens; Drugs, Chinese Herbal; Female; Fibrin; Male; Nephritis, Interstitial; Phenanthrenes; Rats; Rats, Wistar; Stomach Neoplasms

A murine monoclonal antibody, designated 1H10, produced using a human fibrin-related immunogen, was shown to bind avidly to dog fibrin, but not to dog fibrinogen. Using immunofluorescence, fibrin was detected in canine gastric adenocarcinoma and in mixed tissue from a mammary tumour. No fibrin could be detected in bronchogenic carcinoma tissue.

Topics: Adenocarcinoma; Animals; Antibodies, Monoclonal; Dogs; Enzyme-Linked Immunosorbent Assay; Fibrin; Humans; Stomach Neoplasms

A relationship between blood coagulation system and tumor growth is discussed. The role of hemocoagulation disorders in the pathogenesis, dissemination and advancement of disease at molecular, cellular, body and population level is discussed on the basis of the authors' results and literature data. Rastr electron microscopy and immunofluorescent method revealed fibrin on the surface of tumor cells both in vivo and in vitro which is thought to play a masking and protective role. Studies of clotting and anticlotting factors in various subgroups of healthy subjects and patients with precancer and cancer revealed a steady-state increase in blood coagulability in cancer patients of old age. On these grounds, blood fibrinogen level was used for screening. Increased fibrinogen level was associated with higher tumor occurrence. The authors' concept of the pathogenetic role of the coagulation system in tumor growth provided a rationale for the use of direct and indirect anticoagulants in addition to cytotoxic drugs for breast, ovarian and gastric cancer. As a result, cytotoxic treatment was more effective and better tolerated.

Topics: Adult; Anticoagulants; Antineoplastic Agents; Blood Coagulation Disorders; Breast Neoplasms; Cell Line; Cells, Cultured; Drug Therapy, Combination; Female; Fibrin; Fibrinogen; Humans; Lung Neoplasms; Male; Middle Aged; Stomach Neoplasms; Surface Properties; Tumor Cells, Cultured

The investigation of extracts from intact and tumour tissues of the human stomach has revealed that adenocarcinoma tissues possess abnormal antiheparin, antithromboplastin and fibrin-stabilizing activity. Heparin activity, as well as the level of antithrombin III and fibrinogen-heparin complex were sharply reduced. The accumulation of anti-heparin compounds normalizing heparin and displacing the coagulating albumin from the complex with anticoagulant is believed to be one of the most important factors of fibrin production in the tumour.

Topics: Adenocarcinoma; Aged; Blood Coagulation; Female; Fibrin; Gastric Mucosa; Humans; Male; Middle Aged; Stomach Neoplasms

Topics: Adult; Aged; Carcinoma; Fibrin; Fibrin Fibrinogen Degradation Products; Humans; Middle Aged; Neoplasm Metastasis; Plasminogen; Prognosis; Protease Inhibitors; Stomach Neoplasms

Topics: Aged; Blood Coagulation Disorders; Disseminated Intravascular Coagulation; Dysgerminoma; Female; Fibrin; Humans; Male; Middle Aged; Neoplasm Metastasis; Pancreatic Neoplasms; Prostatic Neoplasms; Stomach Neoplasms; Testicular Neoplasms; Thrombophlebitis

Topics: Breast Neoplasms; Colonic Neoplasms; Esophageal Neoplasms; Fibrin; Fibrinogen; Fibrinolysis; Gallbladder Neoplasms; Humans; Lymphatic Metastasis; Neoplasms; Pancreatic Neoplasms; Rectal Neoplasms; Stomach Neoplasms

Topics: Adenocarcinoma; Anemia, Hemolytic; Blood Coagulation Disorders; Diagnostic Techniques, Surgical; Erythrocytes; Fibrin; Humans; Lung; Male; Microscopy, Electron; Microscopy, Electron, Scanning; Middle Aged; Postmortem Changes; Stomach Neoplasms

Topics: Adenocarcinoma; Adenoma; Adrenal Gland Neoplasms; Breast Diseases; Breast Neoplasms; Female; Fibrin; Fluorescent Antibody Technique; Genital Neoplasms, Female; Hodgkin Disease; Humans; Lymph Nodes; Lymphatic Diseases; Melanoma; Methods; Neoplasms; Neoplasms, Nerve Tissue; Ovarian Neoplasms; Sarcoma; Stomach Neoplasms; Thyroid Neoplasms; Tuberculosis

Topics: Fibrin; Fibrinolysin; Geriatrics; Humans; Neoplasm Metastasis; Neoplasms; Skin Neoplasms; Stomach Neoplasms; Tongue Neoplasms; Toxicology