fibrin and Splenic-Diseases

Topics: Adolescent; Adrenal Glands; Adult; Aged; Blood Coagulation Disorders; Child; Female; Fibrin; Hemorrhagic Disorders; Heparin; Humans; Kidney; Male; Meningitis; Middle Aged; Pneumococcal Infections; Sepsis; Splenectomy; Splenic Diseases; Thrombosis; Waterhouse-Friderichsen Syndrome

The aim of the paper was to perform a chronological assessment of the phenomenon of delayed rupture of the spleen, to assess the phenomenological order about the sub-capsular hematoma transformation to determine the causal relationship with trauma as hypothetical cause of death. 80 cases of blunt trauma with splenic capsular hematoma and subsequent rupture of the spleen were evaluated: 38 had an acute rupture of the spleen, 42 presented a break in days or weeks after the traumatic injury. Time between the traumatic event and delayed rupture of the spleen is within a range of time from one day to more than one month. Data recorded included age, sex, type of trauma, injury severity score, grade of splenic injury, associated intra-abdominal injuries, pathologic specimen evaluation. Immunohistochemical investigation of perisplenic hematoma or laceration was performed utilizing polyclonal antibodies anti-fibrinogen, CD61 and CD68, and showed structural chronological differences of sub-capsular hematoma. Expression of modification and organization of erythrocytes, fibrinogen, platelets and macrophages provides an informative picture of the progression of reparative phenomena associated with sub-capsular hematoma and subsequent delayed splenic rupture. Sub-capsular splenic hematoma dating, which we divided into 4 phases, is representing a task in both clinical practice and forensic pathology.

Topics: Adult; Aged; Aged, 80 and over; Case-Control Studies; Erythrocytes; Female; Fibrin; Fibrinogen; Forensic Pathology; Hematoma; Hemorrhage; Hemosiderin; Humans; Immunohistochemistry; Lacerations; Macrophages; Male; Middle Aged; Monocytes; Platelet Aggregation; Retrospective Studies; Spleen; Splenic Diseases; Splenic Rupture; Staining and Labeling; Time Factors; Wounds, Nonpenetrating

Fibrin sealant (FS) is a biologic adhesive containing highly concentrated human fibrinogen that is effective in the face-to-face sealing of tissues, and in establishing hemostasis. We evaluated FS in 32 experimentally produced splenic injuries in six adult mongrel dogs. Complete hemostasis was achieved in all animals prior to closure. The dogs were reexplored postoperatively at intervals varying from four hours to six weeks (mean +/- SD, 21 +/- 20 days). When the dogs were killed, there was no gross evidence of splenic disruption or recurrent bleeding; all of the spleens had developed well-healed capsules. Histologic examination demonstrated a regenerated fibrous capsule extending over the superficial injuries as well as into the deep injuries, without significant inflammatory response. We conclude the following: that FS provides adequate hemostatic control of superficial and deep splenic injuries, FS has good systemic and local compatibility, it can be applied to bleeding parenchymal wounds, it reduces the need for parenchymal sutures that may be traumatic, and it promotes splenic wound healing.

Topics: Animals; Dogs; Fibrin; Hemorrhage; Hemostasis; Splenic Diseases; Tissue Adhesives

Experimental infection in mice with Dermatophilus (D.) congolensis-like microorganisms was carried out, intraperitoneally and subcutaneously. This strain had been isolated from porcine tonsil and reported to be different in some morphological and biological points from D. congolensis. Macroscopic examination revealed multiple abscesses in the peritoneal cavities, or subcutaneous abscesses after the intraperitoneal or subcutaneous injection respectively. Histopathologic examination revealed the characteristic arrangement of the neutrophils surrounding the bacterial colony and peripheral macrophages in the abscess lesions. The lesions contained many microorganisms which showed wide range of the characteristic morphologic variation such as: mycelial elements, coccoid elements and large coccoid elements with transverse or longitudinal septa. Chlamydospore-like elements were sometimes found in the microcolonies in early lesions. The morphology of the lesions and the microorganisms was compared with those of other bacteria including D. congolensis.

Topics: Abscess; Actinomycetales; Actinomycetales Infections; Animals; Fibrin; Granuloma; Inflammation; Liver Abscess; Macrophages; Mice; Necrosis; Neutrophils; Palatine Tonsil; Pancreatic Diseases; Peritoneal Cavity; Splenic Diseases; Swine

Topics: Adult; Arteries; Child; Collagen; Fibrin; Fluorescent Antibody Technique; Histocytochemistry; Humans; Hyalin; Spleen; Splenic Diseases

Topics: Adrenal Gland Diseases; Adult; Afibrinogenemia; Arteries; Cerebral Hemorrhage; Female; Fetal Death; Fetal Diseases; Fibrin; Humans; Infant, Newborn; Liver Diseases; Portal Vein; Pregnancy; Splenic Diseases; Thromboembolism; Umbilical Arteries; Umbilical Cord