fibrin and Rheumatoid-Nodule

Topics: Arthritis, Rheumatoid; Connective Tissue; Fibrin; Humans; Necrosis; Rheumatoid Factor; Rheumatoid Nodule; Synovitis; Wounds and Injuries

Topics: Collagen Diseases; Cysteine; Extracellular Space; Fibrin; Humans; Rheumatoid Nodule; Shwartzman Phenomenon; Tryptophan; Tyrosine

Nodules obtained from five patients with classical seropositive rheumatoid arthritis were studied by an immunofluorescence technique using polyclonal antibodies to IgG, IgA, IgM, C3c, and fibrin, and monoclonal antibodies to the terminal (C5b-9) complement complex (reaction with a neoantigen in C9 revealed during activation), DR antigens, T cells, macrophages, and interdigitating cells. In all instances the central necrotic areas stained strongly for fibrin and more weakly for IgG, IgA, IgM, C3, and terminal complement complex. The surrounding palisading cells reacted with antibodies to DR and macrophages. In the peripheral granulomatous tissue most of the lymphocytes reacted with the antibodies to T cells, whereas various amounts of the larger mononuclear cells were stained by antibodies to DR antigens, macrophages, and interdigitating cells. In all instances the walls of some of the smaller vessels in the granulomatous tissue stained for fibrin, C3, and terminal complement complex. Plasma cells were not seen except for scattered IgM cells in one nodule. These results support the view that the palisading cells are derived from macrophages, and indicate that there is vasculitis with activation of C3 and the terminal complement pathway in the granulomatous tissue.

Topics: Arthritis, Rheumatoid; Autoantigens; Complement C3c; Fibrin; Fluorescent Antibody Technique; HLA-DR Antigens; Humans; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Macrophages; Rheumatoid Nodule; T-Lymphocytes

Eighteen nodules from patients with rheumatoid disease were studied histologically and immunohistochemically. A continuum of microscopic changes was observed with varying degrees of fibrinoid necrosis, mononuclear cell infiltration and healing by fibrous scarring. In two cases there was focal evidence of arteritis. Fibrin was plentiful in the necrotic areas of nodules. Small amounts of immunoglobulin were identified in plasma cells and as irregular extracellular deposits in and around areas of necrosis. In a single small vein abnormal IgG was detected. Mononuclear cells surrounding areas of necrosis stained strongly with antisera to ferritin and a cytoplasmic macrophage antigen, stained variably with muramidase (lysozyme) and negatively with alpha-1 antitrypsin antibodies. Perls' stain for ferric iron was almost entirely negative and ultrastructural x-ray microanalysis indicated that the cytoplasm of these cells were entirely free of iron. These findings confirm the chronic inflammatory nature of rheumatoid nodules but provide no support for the view that they originate in areas of vasculitis. A relative lack of cytoplasmic antiprotease along with a strong expression of ferritin appears to be a characteristic feature of macrophages in rheumatoid tissue.

Topics: Adult; Aged; Blood Proteins; Electron Probe Microanalysis; Female; Ferritins; Fibrin; Histocytochemistry; Humans; Immunochemistry; Immunoglobulins; Iron; Male; Microscopy, Electron; Middle Aged; Monocytes; Muramidase; Rheumatoid Nodule

The presence and localization of fibrin and fibronectin in rheumatoid nodules were studied using an indirect immunoperoxidase technique on tissue specimens fixed in formaldehyde, embedded in paraffin and pretreated with pepsin and testicular hyaluronidase. Three zones characteristic for rheumatoid nodules was recognized. Central area with necrosis, containing at least in part fibrinogen-antigenic material and fibronectin especially in the peripheral part of the necrotic area. Around the necrosis a layer of mesenchymal cells in a palisade arrangement was found. Especially in the external part of this layer fibronectin was demonstrated around and between the cells, where fibrin was absent. Peripherally, a zone of non-specific granulation tissue containing moderate amount of fibronectin decreasing towards the surround mature connective tissue, was seen. In the border of the cellular layer vessels were found in variable amount. In some of the vessels vasculitis was demonstrated with the presence of inflammatory cell infiltration, fibrin deposition and occasionally thrombosis. The pathogenesis of the inflammatory reaction in rheumatoid nodules is discussed.

Topics: Adult; Aged; Female; Fibrin; Fibronectins; Humans; Immunoenzyme Techniques; Immunoglobulin G; Male; Middle Aged; Necrosis; Rheumatoid Nodule; Staining and Labeling

A 33-year-old male with definite Behçet's disease had rheumatoid-like nodules at his elbows. This finding, we believe, represents another manifestation of vasculitis in Behçet's disease.

Topics: Adult; Behcet Syndrome; Fibrin; Humans; Male; Neutrophils; Rheumatoid Nodule; Ulna

Topics: Adolescent; Adult; Aged; Female; Fibrin; Fibroblasts; Glycosaminoglycans; Histocytochemistry; Humans; Male; Middle Aged; Necrosis; Rheumatoid Nodule

Topics: Arthritis, Rheumatoid; Female; Fibrin; Histocytochemistry; Humans; Male; Necrosis; Placenta; Rheumatoid Nodule; Stomach Ulcer; Synovial Membrane

Topics: Allergy and Immunology; Animals; Antigen-Antibody Reactions; Arthritis; Arthritis, Rheumatoid; Fibrin; Haplorhini; Humans; Pathology; Rheumatic Diseases; Rheumatoid Factor; Rheumatoid Nodule

Topics: Chromatography; Fibrin; gamma-Globulins; Gelatin; Peptides; Proteins; Rats; Research; Rheumatic Diseases; Rheumatoid Nodule; Serum Albumin; Trypsin